hemostasis II – Flashcards

Unlock all answers in this set

Unlock answers
question
what is thrombopoietin (TPO)?
answer
the factor for hematopoietic stem cells to go toward toward megakaryocytes
question
how do megakaryocytes produce platelets?
answer
endomitosis, the nucleus is replicated but the DNA remains intact and starts sloughing off cells -> platelets
question
where is TPO produced?
answer
in the bone marrow, liver and kidney. (EPO produced in the kidney)
question
what is the receptor for TPO?
answer
CMPL
question
how are platelets activated?
answer
ADP is released by many platelets and received by P2Y1 and P2Y12 which then through signal cascades changes the conformation of GpIIb/IIIa causing it to bind fibrinogen
question
what does platelet receptor GpIb/V/XI bind?
answer
vWF and endothelium
question
what is glanzmann thrombasthenia?
answer
a deficiency in GpIIb/IIIa, which keeps the platelets from aggregating b/c they have to bind to fibrinogen to bind to each other. (a similar effect would be seen with afibrinogenemia)
question
what is bernard soulier syndrome?
answer
a deficiency in GpIb; therefore platelets cannot adhere to the subendothelium. (similar to vWD, where platelets cannot adhere to the endothelium)
question
what is the specific amino acid sequence both GpIb and GpIIb/IIIa bind to in fibrinogen and vWF?
answer
RGD: arginine, glycine, aspartate
question
what is an additional function of vWF?
answer
carrying/protecting factor VIII - so a vWF is similar to hemophila A
question
what is ADAMTS-13?
answer
a metalloprotease that cleaves vWF (a large multimeric protein produced by endothelial cells), which is itself made in the liver. ADAMTS-13 is the major regulator for vWF size and provides protection against uncontrolled platelet adhesion.
question
how can a deficiency in ADAMTS-13 cause thrombocytopenia? what is this condition called?
answer
an ADAMTS-13 deficiency is called thrombotic thrombocytopenia purpura and in this case, the platelts are all adhering to large vFW fragments on the subendothelium/aggregations and are not present in general circulation
question
what causes ADAMTS-13 to cut vWF into smaller pieces?
answer
high shear stress
question
what two granules do platelets have?
answer
alpha (PF4, vWF, etc) and dense granule (ADP etc)
question
what is grey platelet syndrome?
answer
large platelets that do not have alpha granules
question
where do prostaglandins come from?
answer
phospholipase A2 cuts arachidonic acid from the #2 position, COX on platelets make the prostaglandin TXA2 while COX on endothelial cells will make PGI2(prostacyclin). (TXA2 is one of the most potent vasoconstrictors known)
question
if you eat more omega-6 FA's such as arachidonic acid, what eicosanoids will be produced in greater number?
answer
series 2 prostaglandins (TXA2/PGI2), and series 4 leukotrienes
question
if you eat more omega-3 FA's such as EPA (eicosapentanoic acid), what eicosanoids will be produced in greater number?
answer
series 3 prostaglandins (antiinflammatory), series 5 leukotrienes
question
what omega # is linoleic acid? is there a correlation with cardiovascular disease?
answer
omega 6; of which there is a INVERSE correlation with cardiovascular disease with (it may decrease the risk of CVD)
question
what omega # is linolenic acid? what is it said to do?
answer
omega 3; which should decrease the amount of pro-inflammatory cytokines (TNF-alpha, IL-1, and IL-6) and also decrease CVD
question
what happens to platelet membranes that PLA2 has cleaved all the arachidonic acid from?
answer
the phospholipids missing an arachidonic acids "flip out" causing the surface to be negatively charged - allowing them to ready for coagulation
question
what is found on the surface of platelets when activated?
answer
once activated, integrins form a "surface raft" which help the platelet by allowing for stronger surface interatction/signalling events
question
what are platelet microparticles (PMP)?
answer
these are tiny particles released from cells (including platelets) during apoptosis that have a negative charge which is pro-coagulant/thrombogenic. tissue factor is also found on these microparticles in encrypted form.
question
what is the shape change that occurs when platelets are activated?
answer
round to discoid
question
what is the platelet cytoskeleton composed of?
answer
actin and myosin which help tighten up the shape of activated platelets
question
what are 2 diseases causing defects in platelet adhesion?
answer
bernard soulier disease which is a lack of GpIb/IX/V and vWF deficiency
question
what are 2 diseases causing problems in platelet aggregation?
answer
glanzmann's (missing GpIIb/IIIb) or afibrinogenemia
question
what is wiskott-aldrich?
answer
X-linked mutation in WASP, causing hereditary thrombocytopenia
question
what is an example of a storage pool disease?
answer
grey platelet syndrom
question
what usually causes TTP? what is the platelet cut off where it has to be considered?
answer
ADAMTS-13, but also infection/autoantibodies. 140,000 is the platelet cut-off
question
what is essential thrombocythemia?
answer
a rare myeloid neoplastic disorder where there are too many platelets (platelets are abnormal in size and aggregate quickly and inappropriately), pts w/this tend to bleed slightly more. it can be treated with warfarin long term
question
what does the blood coagulation cascade start with?
answer
the extrinsic, then later it shifts to the intrinsic
question
what part of the coagulation cascade is associated with the phospholipid surface, platelets, and blood vessels?
answer
the intrinsic pathway
question
what part of the coagulation cascade is associated with phospholipids and tissue factor?
answer
the extrinsic pathway
question
what part of the coagulation cascade is associated with thrombin and fibrin clot formation?
answer
the common pathway
question
what is the key to activation of the coagulation system?
answer
thrombin, which provides feedback activations
question
what are the 3 things that can activate factor XI in the intrinsic pathway?
answer
XII, VII and thrombin
question
what 2 things activate factor IX?
answer
factor XI and VII
question
what 2 factors activate factor X?
answer
VII and IX
question
what complex shifts the coagulation cascade to the intrinsic system?
answer
tissue factor pathway inhibitor which inhibits the complex of factor VII, tissue factor, and factor X. increased thrombin concentration then activates the intrinsic side through feedback loops
question
where is tissue factor found in it's inactive state?
answer
tissue factor is an (internal/external) integral membrane protein usually expressed by extravascular cells (esp heart, lungs, testes, uterus, placenta) and encoded on microparticles. it is separated by a primary hemostatic barrier on blood vessels, but following injury it rapidly activates blood coagulation
question
what is the structure of fibrinogen? where does thrombin cleave?
answer
a mustache where alpha/beta/gama are at each end, gamma points down in the middle where the mouth would be and the alpha/beta chains point up into where the nose would be <- where thrombin cleaves, and once i does = fibrin
question
what is a fibrin soft clot?
answer
when thrombin cleaves the center alpha and beta chains, this spot gets sticky and it binds to the ends of other fibrin molecules non-covalently
question
how do soft fibrin clots become hard?
answer
factor XIII crosslinks (ligase between a lysine and a glutamine) adjacent fibrin molecules after being activated by thrombin
question
what are the procoagulant properties of thrombin?
answer
platelet activation (via integrin), activates factors V, VIII as well as XI and VII, it converts fibrinogen to fibrin and activates factor XIII
question
what is the lupus antiphospholipid antibody?
answer
people with this have no (or very minor) bleeding disorder, however, their blood is slow to clot in a test tube and they will have a prolonged PTT (b/c antibodies block the phospholipids which are part of the test). in severe cases, this can be treated with oral anticoagulants
question
how do you determine if someone has phospholipid antibodies?
answer
russel's viper venom activates factor X directly (bypasses blood clotting cascade to test factor X)
question
how do thrombin and endothelial cells inhibit thrombosis?
answer
thrombin is inactivated by antithrombin III, and endothelial cells provide heparin to potentiate this
question
how do endothelial cells inactivate factors X and VII?
answer
via tissue factor pathway inhibitor
question
how do endothelial cells inhibit platelet aggregation?
answer
release of PGI2, NO and ADP phosphatase
question
how do endothelial cells activate the fibrinolytic system?
answer
by releasing tissue plasminogen activator
question
how is protein C activated? what does it do?
answer
protein C is activated when thrombin binds to thrombomodulin as expressed by endothelial cells at the correct time. this complex activates protein C which then inactivates factor V and VIII
question
what does tissue factor pathway inhibitor inhibit?
answer
extrinsic factor X activation, which needs the complex to act
question
what causes factor V leiden? what happens as as result?
answer
arg->gln mutation. the factor V leiden mutation leads to protein C resistance by factor V, leading to more clotting than usual (5-7x increased risk of thromboembolism)
question
what are 2 inhibitors in the thrombolytic system?
answer
plasmin activator inhibitor and plasmin inhibitor <- both will allow the clot to remain/continue to form
question
what are some thrombolytic therapy agents? how are they administered?
answer
streptokinase, urokinase, t-PA (tissue plasminogen activator, alteplase) and recombinant t-PA. these are delievered by catheter to the area of the clot except with pulmonary embolisms (b/c it will dilute the drug and break up other possibly necessary clots)
question
what kind of protein are factors XII, XI, X, II, VII, plasmin, C1, elastase, and trypsin? what inactivates these?
answer
serine proteases which have 3 AAs in their active site; aspartate, histadine and serine. these are inactivated by serpins which are produced in the liver, circulate the blood and suicide inhibit serine proteases and clear them from circulation
question
what are serpins/hemostatic inhibitors that block clotting?
answer
ATIII, C1inh, alpha1 protease inh
question
what are serpins/hemostatic inhibitors that block fibrinolysis?
answer
plasminogen activator inhibitors (PAI), and plasmin inhibitors
question
what are serpins/hemostatic inhibitors that shift reactions?
answer
TFPI shifts from extrinsic to intrinsic
question
what does heparin do (2 things)?
answer
binds to antithrombin III and enhances its inhibitory activity by changing its conformation and folding out and drawing targets in
question
what are benefits of LMW heparin?
answer
higher anti Xa/IIa activity ratio (inhibits thrombin generation, not just thrombin activity), it has a longer half life, it is more uniform/predictive, it may not react with protamine as an antidote, and it has a ****lower association with HIT
question
are there any benefits of unfractionated heparin?
answer
it may be better for obese, elderly, renally insufficient, and pregnant pts
question
does LMW heparin have less of a chance of causing HIT?
answer
yes
question
what is HIT?
answer
a highly prothrombotic, hypercoagulable disorder (not hemorrhagic), caused by exposure to heparin in complex with PF4 on the platelet surface, resulting in an immune rxn -> leading to a systemic thrombotic response (venous 4x more common)
question
what is hirudin?
answer
a direct thrombin inhibitor that is unaffected by platelet factor 4 and inactivates thrombin bound to fibrin -> more predictable anticoagulant response (derived from leeches)
question
what can snake venoms do in terms of coagulation?
answer
snake venom can initiate consumption of coagulant substances and; produce a protease that directly hydrolyses prothrombin, directly clots fibrinogen, activates platelets (kills small mammals by overclotting) and activates endothelial cells to produce a plasminogen activator (fibrinolysis)
question
what happens in DIC?
answer
in disseminated intravascular coagulation, there is consumption of all the platelets and coagulation factors resulting in extreme bleeding somewhere else
Get an explanation on any task
Get unstuck with the help of our AI assistant in seconds
New