Growth of microorganisms – Flashcards
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| bacteria replication type |
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| binary fission |
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| binary fission steps |
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| 1 dna replicated, cell elongates 2 cell wall amd membrane begin to devide 3 cell wall forms completely around dna 4 cells seperate |
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| eukaryotic replication type |
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| mitosis and cytokinesis |
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| mitosis steps |
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| prophase, metaphase, anaphase, telophase |
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| bacterial growth formula |
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Nt = N0 * 2t/T |
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| logarithmic bacterial growth formula |
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| log Nt = t * log 2/T + log N0 |
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| T = |
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| replication/cycle time |
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| Nt = |
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| Number at time t |
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| N0 = |
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| number at time zero |
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| t = |
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| time |
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| lowest T for bacteria |
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| 20 mins |
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| lowest T for protozoa |
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| several hours |
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| T for rapidly growing human cells |
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| 1-2 days |
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| What limits bacterial exponential growth |
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| rapid consumption of nutrients and build up of wastes |
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| replication period and burst size of T even bacteriophage |
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| 1 hour and 100 prions per burst |
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| viral eclipse def. |
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| generation time |
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| PFU stands for |
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| plaque forming unit |
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| plaque |
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| zone of dead cells resulting from virus |
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| lytic cycle growth formula |
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| Nt = N0 * Xt/T |
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| X = |
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| burst size, # progeny releases per cell lysed |
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| budding cycle growth equation |
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| Nt = N0 * at |
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| a = |
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| specific growth rate |
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| HIV specific growth rate = |
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| 5000 |
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| complications of antiviral therapy |
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| by the time clinical symptoms and signs appear, the virus population is huge and possibly out of control and may have caused damage |
