Biology Lab 1060 FINAL EXAM

question

determine the metabolic rate for an insect
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MR = amt O2 consumed (mL)/ [duration of exp (hr) * mass (mg)] ml/mg*hr
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determine population size using the mark-recapture method
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N = M1*T2/M2 M1-# individuals marked and released T2 – total # individuals in the second sample M2 – # individuals in the 2nd sample that were marked
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determine community with the highest or lowest species diversity using a shannon-weiner index
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H = -sum (p*lnp) H= population density p= proportion of each species in the population #6 on quiz 4
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determine anthocyanin concentration for a sample
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Anthocyanin concentration = A530nm – 0.24*A653nm
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determine the population size using a removal sampling method and graph paper
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-individuals are removed from the pop. over a series of sampling periods, w/o releasing them back GRAPH: y-axis = # individuals removed per sampling period x-axis = # individuals previously removed -estimated size of pop. is obtained from the pt. where the line of best fit crosses the XXX-axis
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determine population size using a removal sampling method and regression equation
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-individuals are removed from the pop. over a series of sampling periods, w/o releasing them back -use the regression equation to find the XXXXX intercept -plug in 0 for y -# 4 on quiz 4
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determine community with the highest or lowest species richness from data provided
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-high species richness = lots of diff kinds of species in a community -low species richness = not many diff kinds of species in a community lab manual page 35
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1. identify the tissue category for a specific animal tissue 2. distinguish btwn/among animal tissues that belong to same tissue category 3. distinguish btwn mammalian and other vertebrate tissues
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study animal histology pictures
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identify plant tissues and structures
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-study plant tissue pictures
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function of leaves
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to collect energy from sunlight and convert it into chemical energy via photosynthesis
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function of stems
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to support leaves and to transport water and minerals throughout the plant
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function of roots
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to absorb water and nutrients and anchor the plant. they also store food and nutrients
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1. identify function of plant tissues & structures 2. identify plant organs as characteristic of hydro, meso, or xerophytic plants 3. identify tissue system for specific plant tissues
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plants: structure and adaptations POST LAB
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identify a flower as monocot or eudicot
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monocot – floral parts are in multiples of 3 eudicot – floral parts are in multiples of 4 or 5
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identify a flower as perfect or imperfect
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perfect – has both stamen and carpels (male & female reproductive structures) imperfect – only has one or the other
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identify a flower as complete or incomplete
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complete – has all 4 floral parts (sepals, petals, stamen, carpels) incomplete – is missing 1 or more of the 4 floral parts
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identify the parts of a flower
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look at the diagram on page 45 of lab manual
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identify the function of each part of a flower
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study flowers POST LAB starred questions
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determine the concentration of protein in a sample using a standard curve
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y is the absorbance (if you’re given a regression equation) -Absorbance is on the y axis of the standard curve
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determine the per gram concentration of protein for a plant tissue sample
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-use absorbance values on a standard curve or in a regression equation to get protein conc (mg/dL) -divide the concentration by mass of plant tissue to get per gram concentration (mg/dL*g)
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identify the lightest and heaviest proteins on a gel
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the lightest move the farthest and the heaviest move the shortest distance
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relate migration rate of a protein in a gel to the size of the protein
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larger, heavier proteins do not migrate as fast or as far smaller, lighter proteins migrate faster and farther
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interpret correlation data that include r2 values
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0-0.19 very weak 0.2-0.39 weak 0.4-0.69 modest 0.7-0.89 strong 0.9-1.0 very strong
question

describe how to use a spectrophotometer
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-place your solution into a cuvette -make a blank identical to soln but w/o the solute that absorbs light -set the spectrophotometer to the wavelength you want to measure absorbance at (let it warm up before use) -use the blank soln to blank the spectrophotometer -insert cuvette w/ arrow pointing towards the light, and close the chamber -press \”absorb\” -record data
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determine the size of a microscopic organism using calibration provided
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# 2o on midterm
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identify the tissue system for specific plant tissues: Ground, Vascular, and Dermal
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Ground – parenchyma, collenchyma, sclerenchyma Vascular – xylem, phloem, veins Dermal – epidermis, endodermis, cuticle
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describe how to prepare a 1X buffer soln from a concentrated stock buffer solution
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C1V1=C2V2 (10X) V1 = (1X) V2 look at bookmarked website
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determine activity of an enzyme by plotting absorbance data obtained using a spectrophotometer
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the x axis shows time the SLOPE is the ACTIVITY of an enzyme
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identify plant organs as characteristic of hydrophytic, mesophytic, or xerophytic plants
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study differences on plant notecards & pictures
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identify effects of cold temperature on plants
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-cold stresses increase peroxidase activity in many plants -cold stresses cause plants to alter the lipid composition of their membranes in order to prevent them from solidifying; this is done by increasing the amt. of unsaturated phospholipids in the membranes
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major morphological difference between mammalian blood and reptilian blood
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mammalian blood cells are not nucleated, but reptilian blood cells ARE nucleated
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major morphological difference between elastic cartilage and hyaline cartilage
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elastic cartilage has elastic fibers, while hyaline cartilage does not
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which tissue category/group is characterized by a matrix in which cells and fibers are embedded?
answer

connective
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major morphological difference between mammalian bone tissue and amphibian bone tissue
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mammalian bone tissue has a haversian system but amphibian bone does not
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what was responsible for the movement of the dye in the pipette of the respiromtry apparatus when testing oxygen consumption of red flour beetles ?
answer

decreasing levels of gas in the chamber
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determine hemoglobin concentration qualitatively
answer

add diaminofluorine DAF indicator which will turn blue in the presence of hemoglobin

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