Exam 2 – Microbiology Questions And Answers – Flashcards
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| list the pathogenic amoeba |
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| Entamoeba histolytica/dispar, Entamoeba hartmanni, Entamoeba coli, Endolimax nana, Iodamoeba butschlii, Acanthamoeba and Naegleria |
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| lsit the pathogenic flagellates |
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| Dientamoeba fragilis, Giardia lamblia (duodenalis), Chilomastix mesnili, Trichomonas hominis, Trichomonas vaginalis |
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| list the pathogenic ciliates |
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| Balantidium coli |
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| how to ID E. histolytica/dispar |
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| ingested RBCs |
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| how to ID E. hartmani |
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| troph is 4-12 uM and no RBCs |
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| how to ID E. coli |
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| course uneven chromatin with large eccentric karyosome |
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| how to ID Endolimax nana |
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| large and irregular karyosome with no peripheral chromatin |
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| how to ID Iodamoeba butschlii |
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| large glycogen vacuole in cyst |
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| how to ID Acathamoeba |
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| free-living |
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| how to ID Naegleria |
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| free-living |
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| Sarcodina life cycle |
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| ingestions of infective cysts, excystation in small intestine, multiplication of trophozoites in large intestine, passage of trophozoites and cysts in feces |
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| Pseduopod |
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| protoplasmic extension of the trophozoite; allows to move & engulf food |
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| Chromatin |
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| basophilic nuclear DNA |
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| Karyosome |
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| dot or mass of chromatin within the nucleus |
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| Chromatoidal body |
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| rod-shaped structure of condensed RNA material within the cytoplasm of some cysts |
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| Identification of trophozoites characteristics |
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| Size, motility, nuceli #, peripheral chromatin, karyosome, cytoplasm appearance and inclusions |
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| Identification of cysts characteristics |
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| Size, nuclei #, peripheral chromatin, karyosome, cytoplasm inclusions and glycogen |
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| E. histolytica/dispar trophozoite size |
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| 15-25 uM |
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| E. histolytica/dispar trophozoite nucelus |
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| even, peripheral chromatin, small central karyosome |
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| E. histolytica/dispar trophozoite cytoplasm |
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| finely granular, ingested RBCs |
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| E. histolytica cyst size |
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| 10-20 uM |
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| E. histolytica cyst nuceli |
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| 4, even, peripheral chromatin, small central karyosome |
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| E. histolytica cyst cytoplasm |
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| chromatoid body, rounded, elongated |
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| E. hartmanni trophozoite size |
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| 4-12 uM |
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| E. hartmanni trophozoite nucleus |
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| 1, fine, even peripheral chromatin, small central karyosome |
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| E. hartmanni trophozoite cytoplasm |
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| finely granular, no RBCs |
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| E. hartmanni cyst size |
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| 5-10 uM |
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| E. hartmanni cyst nuclei |
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| same as trophozoite, 4 |
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| E. hartmanni cyst cytoplasm |
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| no glycogen, chromatoid body, elongated, rounded ends |
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| E. coli trophozoite size |
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| 15-50 uM |
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| E. coli trophozoite nucelus |
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| 1, chromatin is course and uneven, and karyosome is large and eccentric |
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| E. coli trophozoite cytoplasm |
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| vacuoles, ingested bacteria |
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| E. coli cyst size |
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| 15-25 uM |
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| E. coli cyst nuclei |
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| 8, same as trophozoites |
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| E. coli cyst cytoplasm |
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| no glycogen, chromatoid bodies, elongated, splintered ends |
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| Endolimax nana trophozoite size |
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| 5-12 uM |
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| Endolimax nana trophozoite nucleus |
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| 1, no peripheral chromatin, karyosome large and irregular |
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| Endolimax nana trophozoite cytoplasm |
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| vaculated, infected bacteria |
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| Iodamoeba butschlii trophozoite size |
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| 6-20 uM |
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| Iodamoeba butschlii trophozoite nucleus |
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| 1, no peripheral chromatin, karyosome is large and blot-like |
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| Iodamoeba butschlii trophozoite cytoplasm |
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| vaculated, ingested bacteria |
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| Iodamoeba butschlii cyst size |
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| 6-15 uM |
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| Iodamoeba butschlii cyst nucleus |
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| 1, same as trophozoite |
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| Iodamoeba butschlii cyst cytoplasm |
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| no chromatoid body, glycogen vacuole |
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| Sarcodina (7) |
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| Entamoeba histolytica/dispar, Entamoeba hartmanni, Entamoeba coli, Endolimax nana, Iodamoeba butschlii, Acanthamoeba and Naegleria |
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| Mastigorphora (5) |
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| Dientamoeba fragilis, Giardia lamblia (duodenalis), Chilomastix mesnili, Trichomonas hominis, Trichomonas vaginalis |
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| Axostyle |
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| the axial rod functioning as a support in flagellates |
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| Flagellum |
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| an extension of the ectoplasm which provides locomotion |
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| Undulating membrane |
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| a protoplasmic membrane along the outer edge of certain protozoa which moves in a wavelike pattern |
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| Identification of trophozoites (4) |
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| size and shape, motility, nuclei, and # of flagella |
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| Identification of cysts (3) |
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| size and shape, nuclei and other features |
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| Dientamoeba fragilis trophozoite size |
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| 5-12 uM |
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| Dientamoeba fragilis trophozoite nuclei |
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| 2, no peripheral chromatin, 4-8 karyosomes with clustered granules |
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| Dientamoeba fragilis trophozoite cytoplasm |
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| finely granular, vacuolated, ingested bacteria |
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| Giardia lamblia (duodenalis) trophozoite size |
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| 9-21 x 5-15 uM |
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| how to ID Dientamoeba fragilis |
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| 2 nuclei in trophozoite with 4-8 karyosomes |
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| Giardia lamblia (duodenalis) trophozoite characteristics |
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| 2 nuclei, sucking disks, parabasal bodies, axostyle |
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| how to ID Giardia lamblia (duodenalis) |
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| sucking disks with nuclei |
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| Giardia lamblia (duodenalis) cyst size |
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| 8-12 uM |
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| Giardia lamblia (duodenalis) cyst characteristics |
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| 4 nuclei, cytoplasm is retracted from the wall, fibrils and flagella |
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| Chilomastix mesnili trophozoite size |
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| 10-20 x 3-10 uM |
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| Chilomastix mesnili trophozoite nucleus |
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| 1, small, central karyosome |
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| how to ID Chilomastix mesnili |
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| spiral groove |
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| Chilomastix mesnili cyst characteristics |
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| 6-10 uM, Lemon-shaped with nipple-like protrusion and 1 Nucleus |
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| Trichomonas hominis trophozoite characteristics |
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| 6-14 uM, 1 nucleus, prominent axostyle, undulating membrane (full length of body) |
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| Trichomonas vaginalis trophozoite characteristics |
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| 7-23 x 5-12 uM, 1 nucleus, undulating membrane (1/2 length of body) |
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| how to ID Trichomonas hominis |
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| undulating membrane full length of body |
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| how to ID Trichomonas vaginalis |
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| undulating membrane 1/2 length of body |
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| Epidemic characteristics of Balantidium coli |
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| worldwide, asymptomatic, and mild-moderate chronic recurrent diarrhea |
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| Balantidium coli life cycle |
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| ingesting of infective cysts, excystation in small intestine, multiplication of trophozoites in large intestine and passage of trophozoites and cysts in feces |
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| Balantidium coli trophozoite size |
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| 40-70 x 50-100 uM |
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| Balantidium coli trophozoite nuclei |
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| 1 macronucleus, 1 micronucleus |
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| Balantidium coli trophozoite characteristics |
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| cytosome, cytopyge and cilia |
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| how to ID Balantidium coli |
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| only ciliate |
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| Balantidium coli cyst size |
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| 50-70 uM |
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| Balantidium coli cyst nuclei |
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| macronucleus, micronucleus |
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| Balantidium coli cyst characteristics |
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| thick cyst wall, +/- vacuoles |
