Biology 2170 Exam 3 University of Toledo – Flashcards
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In plants and algae, what is a by-product of photosynthesis?
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O2
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In the Calvin cycle, what happens to NADPH?
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it is oxidized
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Which of the following best describes the benefit to early photosynthetic organisms gained by having two different photosystems in one cell?
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They could use water as an electron donor
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In addition to chloroplasts, which of the following organelles of eukaryotic cells is believed to have originated by endosymbiosis?
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mitochondria
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Where does the oxygen come from that is produced as a by-product of photosynthesis?
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from breakdown of water
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Which step of the Calvin cycle would be affected first by a lack of NADPH?
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reduction
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ATP production in photosynthesis requires:
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light, electrons, and protons
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Photorespiration occurs because:
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there is more O2 than CO2 in the atmosphere.
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Which of the following is thought to be the first group of organisms able to use water as an electron donor in photosynthesis?
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cyanobacteria
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Which of the following is true concerning the Calvin cycle and light-harvesting reactions of photosynthesis?
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They take place in different compartments of the same organelle.
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Which of the following is/are rubisco substrates?
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CO2, O2, and RuBP
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Chemicals called uncouplers can make membranes permeable to small molecules and ions. What effect might un-couplers have on photosynthesis?
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decreased ATP production because the proton gradient would decrease
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The occurrence of photorespiration _____ CO2 and _____ ATP.
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produces; consumes
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It is thought that the evolution of two photosystems from one likely involved:
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either transfer of genetic material or gene duplication and divergence.
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A researcher is carrying out an experiment where she labels a protein with a fluorescent tag (so she can visualize its location in a cell), and introduces this tagged protein into the lumen of a single thylakoid. She observes her treated cells periodically, and notices that the labeled protein can move between grana, but is never observed in the stroma. What can she deduce?
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The protein likely travels through a common lumen shared by thylakoid membranes and grana, and cannot easily diffuse through the thylakoid membrane.
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Which of the following correctly describes the role of rubisco in the Calvin cycle?
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Rubisco incorporates CO2 from the atmosphere into an organic molecule.
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Xanthophyll pigments:
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prevent formation of reactive oxygen species, reflect yellow light, absorb light energy, convert light energy into heat,
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All cells able to carry out photosynthesis have:
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a plasma membrane.
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For every six CO2 molecules incorporated into carbohydrate molecules, how many triose phosphates can be exported from the chloroplast?
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2
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Photosystem I is a _____ than photosystem II.
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weaker oxidant and a stronger reductant
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During their experiments with chlorella, Calvin and Benson found no free glucose. Why do you think this is?
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Glucose is stored as starch.
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From its ground state, photosystem II can:
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absorb light
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Many photosynthetic prokaryotes are capable of cyclic electron transport only. It is likely that such organisms can _____ but not _____.
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produce ATP; reduce CO2
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Photosystem II and photosystem I differ in:
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H+ production, and electron donors and acceptors.
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Which of the following is responsible for the transport of electrons between photosystem II and photosystem I?
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cytochrome b6f.
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The Calvin cycle includes 15 enzymes, most of which are involved in:
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RuBP regeneration.
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Which of the following compounds is required for the carboxylation step of the Calvin cycle?
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RuBP
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Cyclic electron transport enhances ATP production because:
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electrons are transferred from ferredoxin to plastoquinone.
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In the Calvin cycle, ATP is required to:
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donate a phosphate group to 3-PGA.
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Signal transduction results in:
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a response by the cell.
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Which of the following statements is not true regarding a neurotransmitter?
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Like Delta or Notch, neurotransmitters are usually attached to the cell surface.
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In the context of cell signaling, to what does the term ligand refer?
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a signaling molecule
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What is the function of adenylyl cyclase?
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to form cyclic AMP
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Signaling through receptor kinases:
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takes place in most eukaryotic organisms.
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Growth factors such as platelet-derived growth factor function in:
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paracrine signaling
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What is the cell's likely response to ligand binding to a steroid receptor located in the nucleus?
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a change in gene expression
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How many subunits make up some types of G protein?
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three
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The first step following PDGF binding of the receptor is:
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dimerization.
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In cell communication, which one of the following is a requirement of the responding cell?
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It must have receptor proteins.
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Cell signaling over a long distance is known as:
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endocrine signaling.
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Which type of protein adds a phosphate group to another molecule?
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kinase
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GTP binding occurs on which subunit of a G protein?
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the alpha subunit
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Cellular responses that result from receptor kinase activation tend to:
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involve changes in gene expression.
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Recall from the text the example of cell communication in Streptococcus pneumoniae: the rate of DNA uptake by pneumococcal cells increases sharply when they are at high density due to changes in gene expression brought about by signaling between cells. If the pneumococcal cells did not express receptor protein, how would the rate of DNA uptake be affected when the cells reach high density?
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There would be no change in the rate of DNA uptake.
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Which of the following signaling molecules would you expect to travel the longest distance in the human body?
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testosterone
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Which one of the following is not a cell surface-type receptor?
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steroid receptor
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Inactivation of a cell-surface receptor depends on:
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both the binding affinity of the receptor and concentration of ligand surrounding the cell.
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Many scientists use chemical inhibitors to interfere with normal signaling pathways within eukaryotic cells. If such inhibitors are large, nonpolar molecules, what is the likely method of action of these chemical inhibitors?
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These chemical inhibitors likely bind to receptors and interfere with receptor activation or signal-receptor binding.
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The signaling molecule involved in contact-dependent cell signaling is:
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a transmembrane protein.
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Which type of cell-surface receptor undergoes changes in phosphorylation in response to binding of its ligand?
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receptor kinase
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Cyclic AMP (cAMP) is an example of a(n):
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second messenger.
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Ras is a type of G protein because it:
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is only active when it binds GTP.
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The ability of a specific tissue or organ to respond to the presence of a hormone is dependent on:
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the presence of the appropriate receptors on the cells of the target tissue or organ.
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What is a growth factor?
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a small protein that influences cell growth and division, a signaling molecule involved in paracrine signaling, a soluble molecule that can alter gene expression in a cell
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What determines the specificity of a receptor protein?
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the ligand-binding site
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Ras is most similar to:
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the alpha subunit of three-subunit G proteins.
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A researcher introduces a signal produced by bacteria to eukaryotic cells that she is culturing in the laboratory. Remarkably, she notices that this signal results in an increase in eukaryotic gene expression. How is this possible?
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The signal is either similar in structure to a ligand used by eukaryotes, or this signaling pathway is utilized by both prokaryotes and eukaryotes.
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Notch and Delta are both transmembrane proteins involved in cell communication in the developing nervous system of vertebrate animals. What makes Notch different from Delta?
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Notch is a receptor and Delta is a signaling molecule
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How does an "activated" receptor transfer information into the cell?
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through a conformational change of the receptor
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In the G protein-coupled signal transduction pathway, phosphatases are responsible for the inactivation of:
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the protein target activated by protein kinase A.
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What is the end-result of activating the MAP kinase pathway?
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a change in gene expression
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Many diseases are the result of a problem with cell signaling. Which of the following diseases best exemplifies this fact?
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A specific type of cancer, caused by a truncated receptor that becomes stuck in the activated form.
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The first event that happens after a signaling molecule binds to a receptor is:
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the receptor is activated.
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Imagine that a researcher is studying the embryonic development of mice that do not express the signal molecule Delta. What will likely be true of these mice?
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The mice will have fewer glia compared to their normal counterparts.
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Which is the correct order for these steps in cell signaling?
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receptor activation, signal transduction, response
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Cell signaling over a long distance is known as:
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endocrine signaling
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Which of the following is not an essential element involved in communication between all cells?
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second messenger
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Growth factors such as platelet-derived growth factor function in:
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paracrine signaling.
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A researcher has discovered a new ligand that can pass through the cell membrane and bind to a receptor in the nucleus. Which of the following statements is not likely true of this ligand?
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This ligand likely binds to a transmembrane receptor.
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In order for a G protein to be active:
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the alpha subunit must bind GTP and the beta and gamma subunits must separate from the alpha subunit.
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What is the end-result of activating the MAP kinase pathway?
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a change in gene expression
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Inactivation of a cell-surface receptor depends on:
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both the binding affinity of the receptor and concentration of ligand surrounding the cell.
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Cell signaling between two nerve cells can be thought of as an example of paracrine signaling because:
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the cells are in close proximity.
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Where would you expect to find the receptor for a nonpolar ligand?
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either in the nucleus of the cell or in the cytosol
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Why are some mutations of Ras associated with cancer?
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Ras activates the MAP kinase signal transduction pathway.
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In many signaling pathways, once a signaling molecule binds to a receptor, the receptor becomes phosphorylated. This initial phosphorylation step best demonstrates:
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receptor activation.
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Signaling molecules involved in paracrine and autocrine signaling:
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travel by diffusion.
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Why don't steroid hormones bind to transmembrane cell-surface receptors?
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Steroid hormones are nonpolar and therefore are able to cross the cell's plasma membrane, binding to receptors inside the cell.
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Which of the following signaling molecules would you expect to travel the longest distance in the human body?
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testosterone
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Which of the following structures are responsible for the shape of a cell?
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cytoskeleton
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Dynamic instability is the rapid assembly and disassembly of:
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microtubules.
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Cadherin is an example of:
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a cell adhesion molecule.
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Which of the following is a component of the extracellular matrix made by a plant cell?
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cellulose
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An epithelial tissue is defined as:
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a collection of cells that lines cavities or outside surfaces.
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Which one of the following cytoskeletal elements is associated with supporting the movement of materials inside the cell?
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both microtubules and microfilaments
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Which of the following cell junctions is involved in creating a barrier between cells?
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tight junction
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The extracellular matrix is composed of:
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proteins and polysaccharides
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Predict what would happen if an embryonic epidermal cell switched from expressing E-cadherin to N-cadherin.
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The cell would leave the epidermis and join the neural cells.
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Which of the following is a component of the extracellular matrix produced by an animal cell?
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cellulose
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A researcher is studying a population of cells that are shaped normally, but appear to slide past one another. This is likely the result of mutations in genes encoding:
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components of cell junctions.
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Which one of the following is not a cellular function associated with microtubules?
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muscle contraction
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The most likely reason(s) a metastatic tumor cell might lose its connection to the basal lamina would be:
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the loss of integrin proteins from the cell's surface, or a change in the type of integrin proteins present on the cell's surface.
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Within the plant cell wall, the primary cell wall contains ______, and the secondary cell wall is composed of ______.
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both pectin and cellulose; both lignin and cellulose
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Which of the following statements about the skin is true?
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The epidermis is made up of epithelial tissue whereas the dermis is made up primarily of connective tissue.
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How do cells connect to the extracellular matrix?
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through integrins
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Which of the following is a specific type of extracellular matrix?
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basal lamina
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The basal lamina is:
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a specialized form of the extracellular matrix found beneath all epithelial tissues.
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Which one of the following is not a cellular function of microfilaments?
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flagellar movement
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The natural loss of dead cells from the surface of your skin is a result of:
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both the loss of adherens junctions between epithelial cells, and the breakdown of desmosomes.
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In nerve cells, the centrosome is located near the nucleus. Vesicles move from near the nucleus to the end of long extensions (axons) along microtubules. Based on these two facts, it is possible to conclude that these vesicles are moving in the _____ direction on the microtubule using the motor protein _____.
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plus end; kinesin
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A researcher is assessing the expression of a certain protein in cells. This protein appears as a "web" in the cytoplasm of cells. The protein is likely:
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a component of cytoskeleton
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Which of the following cytoskeletal elements is capable of assembling and disassembling in the cell?
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both microflaments and microtubules
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Macrophage cells undergo a process called phagocytosis in which material is brought into a cell in the form of membrane vesicles and then transported along microtubules toward the cell center. Assuming that the centrosome is near the cell center, you can predict that these vesicles are moving to the _____ end of microtubules using the motor protein _____.
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minus end; dynein
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The internal cytoskeletal structure of cilia and flagella grows out from a structure called a basal body located near the cell surface. Based on this information, where would you predict the plus end of the microtubules to be located?
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near the tip of the cilia or flagella
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In skin, the cells of the epidermis connect to the basal lamina through cellular junctions known as hemidesmosomes. Select the cytoskeletal element that helps maintain the integrity of this connection.
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intermediate filaments
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Gap junctions and plasmodesmata have what feature in common?
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They both allow direct transport of materials between cells.
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The process of cell division in a prokaryotic cell is called:
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binary fission.
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Which of the following is not a characteristic of meiotic cell division?
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Daughter cells are genetically identical.
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What is a cyclin?
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a protein that activates kinases and a protein whose levels change with the cell cycle
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A phragmoplast functions to:
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form a new cell wall.
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Another name for non-sister chromatids is:
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homologous chromosomes.
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The division of the cell's cytoplasm in a eukaryotic cell is known as:
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cytokinesis
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In which phase of mitosis does the nuclear envelope reform?
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telophase
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What type of protein is being activated by the G1/S cyclin-CDK complex to promote the expression of histone proteins?
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a transcription factor
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A malignant cancer differs from a benign cancer in that:
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malignant cancers invade surrounding tissue and benign cancers do not.
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The division of genetic material in a eukaryotic cell is called:
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mitosis.
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What is the function of the centromere?
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to attach the sister chromatids to each other
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What would happen if crossing over occurred between sister chromatids?
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Nothing would happen because sister chromatids are genetically identical or nearly identical.
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Which one of the following statements regarding the S cyclin-CDK complex is true?
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It ensures that DNA is not replicated multiple times.
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A woman has her genome sequenced and finds that she carries a mutation in p53. Will she develop cancer?
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Possibly—this mutation may predispose her to developing certain types of cancer.
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Replication of DNA in a eukaryote occurs during which phase of the cell cycle?
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S phase
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At the start of mitosis, how many sister chromatids are present in a human cell?
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92
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Homologous chromosomes separate from each other in:
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meiosis I.
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The division of genetic material in a eukaryotic cell is called:
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mitosis
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In which phase of mitosis do spindle microtubules shorten?
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anaphase
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How must spindle microtubules attach to chromosomes during prometaphase of meiosis I?
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Spindle microtubules from one pole must attach to both kinetochores in a pair of sister chromatids.
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In which phase of mitosis do sister chromatids separate?
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anaphase
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Predict what would happen if crossing over between homologous chromosomes occurred as part of mitosis.
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Daughter cells would not be genetically identical, and they could contain two copies of the same allele.
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How do CDKs promote cell division?
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They phosphorylate other proteins.
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What type of gene is c-src (cellular-src)?
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proto-oncogene
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In which phase of mitosis do chromosomes condense?
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prophase
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Replication of DNA in a eukaryote occurs during which phase of the cell cycle?
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S phase
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During what step in meiosis do the daughter cells become haploid?
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anaphase I
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The division of the cell's cytoplasm in a eukaryotic cell is known as:
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cytokinesis.
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In which phase of mitosis does the mitotic spindle form?
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prophase
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During what step in meiosis do the daughter cells become haploid?
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anaphase I
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What protein forms the contractile ring during cytokinesis in animal cells?
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actin
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What is the function of the centromere?
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to attach the sister chromatids to each other
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In which phase of mitosis does the nuclear envelope reform?
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telophase
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Crossing over occurs during which step in meiosis?
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prophase I
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The division of genetic material in a eukaryotic cell is called:
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mitosis.
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Mitotic cell division is considered asexual because:
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the daughter cells receive DNA from one parent cell, and the daughter cells are genetically identical.
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The division of the cell's cytoplasm in a eukaryotic cell is known as:
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cytokinesis.
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A human cell with a total of 23 chromosomes is:
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haploid.
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A cell that is not actively dividing is in what phase of the cell cycle?
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G0
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hey
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hi
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Which DNA sequence is very alike?
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a pair of sister chromatids
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How are sister chromatids held together?
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by cohesion
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Will a mutation acquired by a bacterium very likely be inherited by all daughter cells?
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yes
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What are two external factors that initiate prokaryotic cell division?
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nutrient concentration and environmental conditions
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What are responsible for the dependance hepatocytes on lamin?
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integrins
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What type of cell junction connects to the basal lamina?
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hemidesosome
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CAM is made up of what?
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cadherins and integrins
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What are different types of cell junctions?
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adherins, desmosomes, tight junctions
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Do microfilaments assemble in a "9+2" array?
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no microtubules do
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Do all eukaryotic cells have all three cytoskeleton elements?
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no, plants do not have intermediate filaments
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Which component of cytoskeleton is hollow in the middle?
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microtubules
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What are the three main components of cytoskeleton? (largest to smallest)
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microtubules, intermediate filaments, microfilaments
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Which two components of cytoskeleton are polar?
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microtubules and microfilaments
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Which two components of cytoskelton help with movement?
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microtubules and microfilaments
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What plays a role in cell shape?
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the cytoskeleton
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What are microtubules made up of?
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tubulin
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what are microfilaments up of?
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actin
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What is the initial carbon input in the Calvin cycle?
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rubisco
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What molecule in oxidized in photosynthesis?
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water
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is fluorescence an example of a chemical reaction?
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yes
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photosynthesis
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a biochemical process for building carbohydrates using energy from sunlight and CO2 taken from the air
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What is the photic zone and who deep does it penetrate?
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photic zone is the area in the ocean for which photosynthesis can occur which extends from the surface to about 100m deep
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What type of reaction is photosynthesis?
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redox
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what is reduction?
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acquires electrons and gains energy
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what is oxidation?
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loses electrons and releases energy
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during photosynthesis why are co2 molecules reduced?
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to form higher-energy carbohydrate molecules
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what does photosynthesis require?
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input of energy from ATP and the transfer of electrons from an electron donor
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where does the photosynthetic electron transport chain take place
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specialized membranes
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ATP and NADPH are needed to synthesize carbohydrates using CO2, what is this process called
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the calvin cycle
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where is the photosynthetic electron chain located
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thylakoid membrane
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thylakoid membranes form structures that resemble flattened sacs, groups of flattened sacs are called ______
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grana
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how are grana connected
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membrane bridges
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what is the region surrounding the thylokoid membrane called
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stroma
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single interconnected compartment inside of the thylakoid membrane
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lumen