Proteins of the Red Blood Cell Membrane Essay Example

of the total amount of protein found in the membrane.Spectrin is a cytoskeletal protein that is long and fibrous, and seems to only be found in erythrocyte membranes (Kruckeberg et al.

1980). Along with actin it forms the erythrocyte cytoskeleton, which maintains the shape of the cell and allows it to bend when it passes through capillaries. Glycoporin is a small transmembrane protein, which sticks out of the surface of the cell (and has most of its weight there). Its function however is not understood (Alberts et al. 2002).

Band 3 has an important function as an anion transporter, which means that the red blood cell can increase its CO2 transporting capacity.There are many other structural membrane proteins, eg. band 4. 1 and Ankyrin, which form complexes with spectrin to maintain the cytoskeleton. SDS-Page electrophoresis (polyacrylamide gel electrophoresis) is a way of separating a mixture of proteins that have different molecular masses. It works by denaturing the protein (with SDS and mecaptoethanol) and running the protein samples in a gel.

The protein with the lowest molecular weight will reach the bottom of the gel first, and proteins with higher molecular weights will take much longer.When the gel is stained bands appear and when a marker is also run in the gel, the molecular weights of the proteins shown by the bands can be determined. It would be expected that many different bands would be present on the gel, especially spectrin, glycoporin and Band 3, since these are major proteins within the membrane. The acetylcholinesterase assay was used to determine the amount of activity of the enzyme acetylcholinesterase (a small amount of which is found in

the erythrocyte membrane) after the membrane had been pre-treated, and determine the whereabouts of the enzyme within the membrane.

The enzyme catalyses the reaction shown below: Acetylcholine Acetate +Choline The pre-treatments were neostigmine (an inhibitor of the enzyme) and papain (a cysteine proteinase), and both of these treatments would be expected to inhibit the enzyme, preventing it from working. Both of these pre-treatments can only act on the surface of the cell membrane, so any proteins on the cytosolic side of the membrane should not be affected.The supernatant samples should show no enzyme activity, because the enzyme is contained within the membrane. The activity of the enzyme was measured using a spectrophotometer and adding acetylthiocholine to the pre-treated samples. Acetylcholinesterase was shown to be working when the yellow colour of thiol was produced in the solution, meaning acetylthiocholine had been split into its component parts.

From the amount of absorbance per minute the activity of the enzyme in each sample could be calculated.