Microbiology Chapter 1 Answers – Flashcards

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Microorganisms
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organisms too small to be seen with unaided eye
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Pathogenic microorganism
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One that is disease causing
very few are pathogenic 
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Microbes' functions
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Decompose organic waste

producers in the ecosystem by photosynthesis

produce industrial chemicals such as ethanol

produce fermented food such as vinegar, cheese, etc.

produce manufacturing and disease preventing products

(e.g. cellulose and insulin)

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Knowledge of microorganisms allows humans to
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prevent food spoilage and disease occurrence; led to aseptic techniques to prevent contamination in medicine and in microbiology laboratories
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Naming and Classifying organisms
"scientific nomenclature";
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established by Linnaeus
each has genus and specific epithet, or "species";
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Escherichia coli
(E. coli);
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Honors the discoverer, Theodor Escherich
describes the baterium's habitat, the large intestine or colon
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Straphylococcus aureus
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clustered spherical cells

describes gold-colored colonies

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Types of Microorganisms
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Algae

Archaea

Bacteria

Fungi

Multicellular animal parasites

Protozoa

Viruses

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Bacteria
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Prokaryotes

Pepidoglycan cell walls

Binary fission

uses organic/inorganic chemicals or photosynthesis for energy

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Archaea
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Prokaryotic

Lack peptidoglycan

Live in extreme environments

Include Methanogens, extreme halophiles, and extreme thermophiles

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Fungi
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Eukaryotes

Chitin cell walls

use organic chemicals for energy

molds and mushrooms are multicellylar, consisting of masses of mycelia

yeasts are unicelluar

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Protozoa
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Eukaryotes

Absorb or ingest organic chemicals

motile via pseudopods, cilia, or flagella

ex) amoeba

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Algae
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Eukaryotes

Cellulose cell walls

photosynthesis for energy

produce molecular oxygen and organic compounds

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Viruses
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Acellular

consist of DNA or RNA core

core is surrounded by protein coat

coat may be enclosed in a lipid envelope

replicate only when they are in a living host cell

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Multicellular Animal Parasites
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Eukaryotes

Multicellular animals

Parasitic flatworms and roundworms are called helminths

Microscopic stages in life cycle

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3 Domains
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Bacteria- Mitochondria and Chloroplasts originates

Archaea

Eukarya- degeneration

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Microbiology History
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Ancestors of bacteria were first life on Earth

First microbes were observed in 1673

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Robert Hooke
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1665
Reported that living things are composed of little boxes or cells 

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Rudolf Virchow
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1858: Said cells arise from preexisting cells

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Cell theory
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All living things are composed of cells and come from preexisting cells.
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Anton van Leeuwenhoek
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1673-1723: described live microorganisms in Letters to the Royal Socity of London. Teeth scrapings, rain water, and peppercorn infusions.

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Spontaneous generation
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the hypothesis that living organisms arise from nonliving matter; a "vital force" forms life.

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Biogenesis
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the hypothesis that living organisms arise from preexisting life

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Francesco Redi Experiment

1668

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3 jars covered with net
3 open jars

showed that: maggots came from flied and the covering was to keep flies out. Biogenesis! 
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John Needham Experiment

1745

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nutrient broth was heated and covered and showed microbial growth, supporting spontaneous generation!

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Lazzaro Spallanzani Experiment

1765

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Sealed flask before heating and no microbial growth supporting biogenesis!

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Louis Pasteur

1861

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Demonstrated that microorganisms are in the air. First proved biogenesis through short necked flasks
then used S-shaped flask to keep microbes out but to let air in. Tilted it and let microbes in and saw growth 
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Golden Age of Microbiology
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1857- 1914: Begun with Pasteur's work
discoveries included the relationship between microbes and disease, immunity, and antimicrobial drugs.;

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Fermentation
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-the conversion of sugar to alcohol to make beer or wine
-Pasteur showed that microbes are responsible for this... and spoilage of food

- Found that spoilage could be killed by heat that couldnt evaporate wine or alcohol

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Pasteurization
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the application of a high heat for a short time
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Agostino Basi
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1835: Showed that a silkwork disead was caused by fungus

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Pasteur
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1865: another silkworm disease was caused by protozoan

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Ignaz Semmelweis
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1840s: advocated handwashing to prevent transmission to puerperal fever from one obstetrical patient to another

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Lister
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1827-1912: used phenol to prove that surgical wound infections were caused by microbes

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Robert Koch
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1876: proved that a bacterium causes anthrax and provided the experimental steps, "Koch's postulates", to prove that a specific microve causes a specific disease.

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Edward Jenner
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1796: inoculated a person with cowpox virus and protected them with it.
VACCINATION;

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Vaccines today
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produced from avirulent microbes, or genetic engineering
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Chemotherapy
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treatment with chemicals

antibiotics or synthetic drugs

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Antibiotics
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Chemicals produced by bacteria and fungi that inhibit or kill other microbes
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Quinine
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from treebark, used to treat malaria
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Paul Ehrlich
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speculated about a magic bullet that could destory a pathogen without harming the host.

-Developed a synthetic arsenic drug, salvarsan, to treat syphilis in 1910.

-Sulfonamides were synthesized in the 1930s

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Alexander Fleming
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1928: Discovered first antibiotic, penicillin, that killed S. aureus;
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1940s: Penicillin was tested clinically and mass produced

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Bacteriology
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study of bacteria
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Mycology
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study of fungi
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Virology
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study of viruses
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Parasitology
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study of protozoa and parasitic worms
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Immunology
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-Study of immunity

-Vaccines and interferons are being investigated to prevent and cure viral diseases

-Proposed by Rebecca Lancefield in 1933 (streptococci)

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Microbial genetics
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the study of how microbes inherit traits

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Molecular biology
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the study of how DNA directs protein synthesis
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Genomics
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the study of an organism's genes; has provided new tools for classifying microorganisms

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Recombinant DNA
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DNA made from two different sources
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Berg
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1960s: inserted animal DNA into bacterial DNA and the bacteria produced an animal protein.
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1941 Beadle and Tatum
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genes encode cell's enzymes
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1944 Avery, McLeod, McCarty
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DNA is hereditary material
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1961 Jacob and Monod
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role of mRNA in protein synthesis
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Microbial Ecology
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Bacteria recycle carbon, nutrients, sulfur, and phosphorus that can be used by plants and animals

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Bioremediation
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Bacteria degrade organic matter in sewage

Bacteria degrade or detoxify pollutants such as oil and mercury

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Biological Insecticides
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alternatives to pesticides, prevent insect damage

Bacillus thuringiensis infections are fatal in many insects but harmless to other animals

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Biotechnology
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the use of microbes to produce foods and chemicals

-recombinant DNA technology

-gene therapy

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Microbiota
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normally present in and on human body

prevent growth of pathogens

produce growth factors (Vitamin K ; folic acid)

Resistance (skin, stomach acid, and antimicrobial chemicals)

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Biofilms
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Microbes attach to solid surfaces and grow into masses

grow on rocks pipes teeth and medical implants

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Emerging infectious diseases

(EIDs)

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new or changing diseases that have potential for increasing or are increasing in incidence

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