micro chap1 – Flashcards

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  1. Scientific nomenclature assigns each organism 2 names:

______________ and ______________.

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Genus and species
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Name 7 types of microorganisms.

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Bacteria, Archae, Fundus, Protozoa, viruses, Microscopic algae, Multicellal parasite
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Bacteria divide by
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Binary fission
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Bacterial cell walls contain
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Peptidoglycan
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  1. The 3 domains are _____________, ______________, and _____________.

 

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Bacteria, Archae, Eukarya
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  1. Spontaneous generation says that living organisms arise from _____________ ________________.

 

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Non living matter
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  1. Biogenesis means that living organisms arise from _____________ __________.

 

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Pre-existing life
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  1. _________________ finally disproved spontaneous generation.

 

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Louis Pasteur
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  1. _________________ saw “animacules”.

 

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Anton Van Leeuwenhoek
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  1. The __________ _____________ of _______________ stated that a disease might be caused by a microorganism.

 

 

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Germ theory of disease
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  1. ________________ is the process that heats liquids just enough to kill most of the bacteria that cause spoilage.

 

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Pasteurization
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  1. __________________ used carbolic acid to prevent postoperative infection.
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Lister
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  1. __________________ advocated hand washing to prevent the spread of disease.
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Semmelweis
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  1. __________________ established a sequence of experimental steps for proving that a specific organism causes a specific disease.
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koch
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  1. These steps are known as _________ ______________.
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koch postulates
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  1. The first vaccination was for ____________ by _____________.
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edward jenner
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  1. ___________________ found a cure for syphilis.
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Ehrlich
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  1. ___________________ discovered the first antibiotic.
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flemming
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  1. ___________________ is the study of bacteria.
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bacteriology
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  1. ___________________ is the study of fungus.
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mycology
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  1. ___________________ is the study of protozoa and parasitic worms.
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parasitology
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  1. ___________________ is the study of immunity.
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immunology
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  1. ___________________ is the study of viruses.
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virology
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  1. __________________, _________________, _________________ and __________________ are beneficial activities of microorganisms.
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Microbial ecology, Bio remediation, Biological insecticides, biotechnology
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__________________ ____________________ is a term that describes a variety of microorganisms on and inside our bodies that do us no harm
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Normal Microbiota
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  1. A complex aggregation of microbes is called a _______________.

 

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Biofilms
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  1. _______________ ________________ __________________ or EIDs refer to new diseases and diseases increasing in incidence.
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Emerging Infectious Disease
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  1. ________ developed the formal system of classifying and naming organisms.

 

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Carolus Linnaeus
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  1. A disease causing microorganism is called a ______.
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pathogen
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  1. In a ________________ microscope, the object appears dark against a light background.

 

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Light
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  1. In a ________________ microscope, the object appears light against a black (dark) background.

 

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Dark
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  1. With a _____________ _____________ microscope, detailed examination of internal structures of living organisms is possible.

 

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Phase contrast
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  1. In a _______________ microscope, UV light is used.
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Fluorescent
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  1. In an ____________ microscope, a beam of electrons is used instead of light.
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Electrons
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  1. 3 categories of stains are ___________, _______________, and ____________.
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Simple, differential and special
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  1. With the _____________ _____________ (a differential stain), bacteria can be classified as Gram Positive or Gram Negative.
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Gram stain
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  1. Gram Positive bacteria have a ___________color.
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purple
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  1. Gram Negative bacteria have a ___________ color.
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red/pink
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  1. The correct order of reagents in the Gram Stain are as follows:

_______________

_______________

_______________

_______________

 

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Cristal violet
iodine
Decolorizant agent
Safranin
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  1. List the appearance of gram-positive bacteria after each step in the gram stain:

Crystal Violet: ________

Iodine: ______________

Decolorizer: __________

Safranin: ____________

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purple
purple
purple
purple
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  1. List the appearance of gram-negative bacteria after each step in the gram stain:

Crystal Violet: ________

Iodine: ______________

Decolorizer: __________

Safranin: ____________

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purple

purple
colorless

red

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  1. Three basic shapes of bacteria are _________, ___________ and _________.
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Bacillus, Coccus, spiral
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  1. The function of the capsule (glycocalyx) is to _______ ___________.
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Prevent phagocytosis
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  1. Some bacteria have ____________ which are used for movement.
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Flagella
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  1. _____________ and ______________ are hairlike appendages.

 

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Fimbrae

Pili

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    1. ____________ are used for attachment.

 

 

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Fimbrae
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____________ are used to transfer DNA.
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Pili
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  1. The _________ __________ maintains the shape of the cell.

 

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Cell wall
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  1. The ____________ _____________ allows movement of molecules in and out of the cell.
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plasma membrane
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  1. The ____________ is where chemical reactions occur within the cell.
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cytoplasm
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  1. The ____________ is where chemical reactions occur within the cell.
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cytoplasm
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  1. The _____________ contains the bacterial DNA.
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nucleoid
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  1. ______________ are used for storage.
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inclusions
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  1. ______________ are a “resting state” for some bacteria.
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endospores
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  1. ______________ are pieces of DNA that are independent of the cell’s chromosomal DNA.
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plasmids?
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  1. The requirements for bacterial growth are _______, ________, _______, ____________, and _____________.
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Temperature

PH

Osmotic pressure

atmosphere

chemical requierement

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  1. __________ __________ refers to bacteria that will grow in the presence of oxygen.

 

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Obligate aerobe
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  1. _________ _________ refers to bacteria that will not grow in oxygen.

 

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obligate anaerobe
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  1. ____________ refers to bacteria that will grow in the presence or absence of oxygen.
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Facultative
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  1. ____________ refers to bacteria that will grow in lower levels of oxygen.
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Microaerophilic
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  1. ____________ refer to bacteria that require CO2 in amounts higher than found in the atmosphere.
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capneic
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  1. __________, _____________, ___________ and _____________ are the stages of the bacterial growth curve in the correct order.
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lag

logarithmic

stationary

death

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  1. ________________ is the phase of little activity, plentiful nutrients, and little waste.

 

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Lag
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  1. ________________ is the phase where cellular reproduction is most active.
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logarithmic
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  1. ________________ is a phase of equilibrium where the living cells equal the number of dying cells, nutrients are nutrients are being depleted, and waste is accumulating.
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Stationary
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  1. _______________ is the phase where dying cells outnumber living cells, nutrients are depleted, and the environment is toxic.
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death
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  1. The generation time is 1 hour.  Two cells will become ________ cells after 6 hours.
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24
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An experiment began with 4 cells and ended with 128 cells. How many generations did the cells go through?
A) 64
B) 32
C) 6
D) 5
E) 4
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D
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Site of proteins synthesis
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Ribosomes
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Resistant to processes that kill vegetative cells
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Endows poresEndows pores
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Most human bacterial infections involves
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Biofilms
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The two purines are
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Adenine and guanine
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The two pyrimidines are
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Thymine, cystosine
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Adenine always binds with
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Thymine
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Cystosine binds with
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Guanine
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Three type of rnamRna
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mRNA, tRNA, rRna
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Base substitution of a single base results inmissense
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Miss sense mumutation or

nonsense mutation 

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On or more nucleotides pairtransferred deleted or inserted result in
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Frameshift mutation
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2 common mutagens
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Chemicals, radiation
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Genes are transferred from one bacterial cell to another
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Transformation
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Genetic material directly transferred from one bacterium to another in form of plasmids
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Conjugation
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Bacterial DNA transferred from one bacterial cell to another one inside a virus
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Transduction
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Takes the DNA code out of the nucleoid to the ribosomes
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MRna
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Transport amino acids to the ribosomes
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tRNA
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Study of genes?
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Genetics
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Segment of DNA coding functional product, usually a protein
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Gene
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Structure containing the DNA 

structure containing the genes

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Chromosome
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Cell genetic information?
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Genome
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study of genome
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Genomics
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Gene of organism
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Genome
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Expression of the genes
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Phenotype
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Basic unit of the DNA molecule
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Nucleotide
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Genetic information in DNA copied into complementary based sequence RNA
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Transcription
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Info encode in RNA used to synthesize specific proteins
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Translation
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DNA RNA difference?
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RNA ribose, single strand, contain uracil

dna deoxyribose, helix/ double strand

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Three base in sequence code is
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Codon
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# codon per organism
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64 coding 24 amino acids
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Cause Permanent change in the base sequence of DNA ?
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Mutation
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Different kind of mutations?
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NeutralNeutral, beneficial, harmful
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Base mutation of single base replaced by another base?
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Point mutation
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One or more nucleotide pairs deleted or inserted during mutation?
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Frameshift mutation
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Exchange of genes during 2 DNA molecules to obtain a new combination
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Genetic recombination
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