IB Biology SL Nature of Science + Option: Ecology paper 3

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Discovery of Cyclins
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Tim Hunt and Joan Ruderman were studying gene expression in early embryos. While doing so they found three proteins that varied in concentrations at different times of the cell cycle. These were eventually called cyclins. This illustrates that scientists must always be observant, to spot unplanned and surprising discoveries. This example of 'accidental' discovery is common in science.
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Meselson and Stahl Discovery
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DNA is semi-conservative (Has one old and one new strand)
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Meselson and Stahl experiment
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Two cultures of E.coli were grown, one with 'heavy' isotopes of nitrogen (^15N) and one with normal isotopes (^14N). After many generations of breeding with these two cultures the DNA of the offspring showed one strand of the 14N and one of the 15N proving DNA is semi-conservative
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Epidemiology and causes of lung cancer
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It is a cancerous growth prone to spreading (metastasising). Caused by one or more carcinogens that enter the lungs in cigarette smoke and other fumes. This cancer has a high mortality rate and must be caught early. It's the leading cause of death in the U.S. Other risk factors include radiation, air pollution, previous lung disease, genetics.
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Symptoms of lung cancer
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Short breath, coughing with phlegm, shoulder ; chest pains/aches. Loss of appetite, fatigue and weight loss.
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Epidemiology
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the study of the distribution and determinants of health- related states or events (including disease), and the application of this study to the control of diseases and other health problems.
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Light microscope vs. Electron microscope
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Light: Cheap, simple, 2000x magnification, dead or alive. Electron:expensive, complex, 500,000x magnification, dead
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How are genes read?
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Gene sequencers
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What is the Human Genome project?
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A 13 year effort to find and read the human genome.
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What are key advances in technology for biology?
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PCR, computers to automate sequencing and read genome, fluorescent labelling so all four bases can be analysed at the same time, digital cameras to read dye markers.
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how does PCR work?
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the DNA is multiplied many times in a thermocycler to get millions of copies that can then be analysed
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What was used to discover DNA molecules in chromosomes?
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Autoradiography
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What is autoradiography?
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A process where radiation from a substance is captured on a camera.
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How did John Cairn's use autoradiography?
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He injected radioactive form of Thymidine (radio marker) into DNA to expose the film. He did this to show how a bacterium's chromosome is made up of a single circle of DNA and then took photos of the DNA undergoing replication of the base pairs splitting.
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Why do hydrogen bonds form between water molecules
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Due to the cohesive nature of water, this creates a surface tension useful for some organisms e.g Water skaters
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Cohesive properties of water
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Water is attracted to itself, positive end attracts the negative end and they combine. When frozen the bonds freeze and lock up creating ice, also explains water droplets, water skaters and how water moves up plant stems.
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Adhesive properties of water
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This is when water molecules attach to plant stems (non-water molecule)
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Solvent properties of water
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helps other polar molecules with transportation
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Thermal properties of water
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Acts as a heat stabiliser in organisms, a large change in temperature is required to change the temp of an organism. Also can cool by sweat production in humans.
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Energy flow in ecosystems
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Energy is not constantly recycled . The cycles of growth, death, and decomposition show how nature recycles nutrients, but energy pyramids show that energy flows through a system and is lost. This is why new energy must arrive in the form of sunlight in order to keep the system going.
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How much energy is lost at each trophic level?
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90%
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Explain how natural selection isn't just by chance
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Natural selection is a multi-step process. Steps involve chance, such as variation, or which gametes participate in fertilisation etc. The characteristics in a population is not purely up to chance. For example, falcons with excellent vision or dolphins that are capable of echo-location. It's no coincidence that flowers have adaptations suited to their insect pollinators, or that certain bacteria become resistant to the antibiotics we try to fight them with. Natural selection favours useful adaptations and selects against harmful ones in a way that is not based on chance, but on fitness. Heritable changes are passed on from generation to generation, and accumulate over time so that each population either fits its environment, adapts accordingly, or dies out.
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How Staphylococci bacteria was tested for resistance
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the bacterium was introduced into a Petri dish along with strips of paper that has been soaked in different types of antibiotic. When the colonies of bacteria grow close to the disks, they show resistance to the antibiotic, when wide, circles with no bacteria are present, they show that the antibiotic is stopping the bacteria the way it should.
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Uncertainties to cell theory
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Recent findings that have raised questions about cell theory include observations of striated muscle, giant algae, and aseptate fungal hyphae.
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Striated muscle
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skeletal muscle is composed of muscle fibres, these are long fibres that can measure 300mm or more and are therefore much larger than regular cells. In addition to this, each muscle fibre contains hundreds of nuclei
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Giant algae
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These organisms are able to grow up to 100 mm in length yet they are unicellular and contain only one nucleus. Due to their size one would expect them to be composed of many cells.
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aseptate fungal hypae
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fungi are made of thread like structures called hyphae (normal in fungi) divided by septa. However in aseptate fungi these hyphae are not divided into sections, they are uninterrupted tube like structures containing many nuclei
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Falsification of Vitalism
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Friedrich Wöhler made a discovery that falsified Vitalism. Wöhler mixed two inorganic substances, cyanic acid and ammonium. He noticed the formation of urea. He had previously only come across urea crystals in the study of the compounds that are characteristic of urine. For the first time in a controlled setting, an organic molecule was synthesized from inorganic substances. This was soon used as evidence against vitalism Soon after, substances such as amino acids were synthesized from inorganic precursors in various laboratories.
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Reclassification of the figwort family
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The plant was classified into specific categories that included the family Scrophulariaceae, the figwort family. DNA sequencing was done on many species including foxgloves, and it was determined that some plants did not belong with the other figworts but instead belonged in the family Plantaginaceae along with the plantains. Foxgloves now have a new family.
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Discovery of the circulation of blood
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William Harvey determined that the heart acts as a double pump and that the blood is continuously circulated to/from the lungs and to/from the body. Before it was believed that blood was constantly being used up by the body.
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Non-standard amino acids in proteins
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Most, but not all, organisms assemble proteins from the same 20 amino acids . If you include all known living organisms then there are 22 amino acids that are used to create polypeptides. The additional two are called selenocysteine and pyrrolysine.
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Are all plants/algae autotrophic?
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Most but not all
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Pentadactyl limbs in vertebrates
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Darwin explained that homologous structures were not just a coincidence but evidence that the organisms in question have a common ancestor.
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Monitoring the change in atmospheric gases
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Layers found in sheets of ice formed by annual snowfall can be used to study the substances trapped in the layers, such as air bubbles from the year when the layer was deposited. Researchers have collected layers 3000m down, with climate information 400 000 years old. One indication of temperature is the frequency of different types of isotopes found in the air bubbles. Oxygen atoms are usually found in their most abundant isotope, oxygen-16, but can also be found in their 'heavier' form, oxygen-18, which has two extra neutrons. When glaciations happen, the oceans have a slightly higher ratio of oxygen-18, and the glaciers that form have a slightly higher ratio of oxygen-16. By examining these ratios in ice and in the shells of marine fossils, climatologists can trace the colder and warmer periods of the past.
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Osmosis experiments
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They show that accuracy in experiments is particularly important.
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Why is repetition important in experiments?
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to ensure reliability.
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Gregor Mendel pea experiment
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Used artificial pollination in a series of experiments by choosing the pollen of various plants to fertilize other individuals of the same species. He used a small brush to place the pollen on the reproductive parts of the flowers, thus replacing the insects that do it naturally. This technique takes away the role of chance because the experimenter knows exactly which plants are fertilized by which pollen.
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Mendels results
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F2 of Round × wrinkled seeds= 2.96:1,F2 of Yellow × green seeds=3.01:1, F2 ofGreen × yellow pods= 2.82:1, F2 of Tall × short plants= 2.84:1
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Why is bi-nomial nomenclature used?
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groups of scientists: scientists use the binomial system to identify a species rather than the many different local names.
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How is bi-nomial nomenclature written/typed?
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the genus name is capitalized but the species name is not, both are written in italics when typed, or underlined when handwritten,
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Ethics of stem cell research
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In embryonic stem cell treatment the embryo has to be destroyed, since the embryo is becoming a person some people think it is misusing the gift of life. Before 14 days an embryo is not considered a person as it has no nervous system and can still split to form twins. Taking stem cells from an embryo can be considered stopping it normal development into becoming a human.
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Ethics of Using invertebrates in respirometers
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It is tempting to place invertebrates in respirometers to determine oxygen consumption. However, the use of invertebrates in such experiments has ethical implications. It is a living animal and may cause harm to it.
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Risk of g-mods on the environment
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In 1999 a group of researchers carried out a study to find out if the pollen from genetically modified Bt corn could have a negative effect on the larvae of the monarch butterfly. The study was immediately criticized by some members of the scientific community, who claimed that the quantities of transgenic pollen placed on the caterpillar's food was of a concentration that would not be possible in nature, and that more realistic experiments would need to be carried out in the field.
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Florey and Chain experiment
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In 1929 he discovered mould growing on a glass dish in his laboratory which appeared to kill the bacteria he was cultivating. In his follow-up studies, the crude penicillin broth that he had extracted from the mould was non-toxic to rabbits and mice but acted slowly and disappeared quickly
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Health claims about lipids in the diet
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Some lipids are good such as vitamins, saturated fats increase cholesterol, unsaturated fats are good, should make up 10-25% of the daily intake, animal fats are bad.
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Effects of climate change as a result of humans
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More intense and frequent droughts, Flooding, rising sea levels, less food production, more disease, loss of biodiversity, extreme climate conditions
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How to set up a Mesocosm
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Can be in open tanks, but sealed glass vessels are preferable because entry and exit of matter can be prevented but light can enter and heat can leave. Aquatic systems are likely to be more successful than terrestrial ones.
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How would a quadrat be used by an ecologist?
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To estimate the sizes of plant populations by random sampling
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What components are needed to make an ecosystem?
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A community and its abiotic environment
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Biotic factors
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attributes in an ecosystem that refer to living organisms.
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Quadrat sampling
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square or rectangular plot of land, a quadrat, marked off at random to isolate a sample and determine the percentage of vegetation and animals occurring within the marked area.
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Sustainable communities
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communities that are capable of being maintained at a steady level without exhausting natural resources or causing severe ecological damage.
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Food chains
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a feeding hierarchy in which organisms in an ecosystem are grouped into trophic (nutritional) levels and are shown in a succession to represent the flow of food energy and the feeding relationships between them.
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Light energy
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the energy produced or given off directly from the sun causing the growth of plants and the existence of most life forms.
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Pyramids of energy
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a graphical model to show how the energy flows through a food chain, how the amount of energy is decreasing and becoming less available for organisms as it enters each trophic level, and how much of the energy in the ecosystem is lost to the atmosphere as heat.
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Biomass
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the total mass of living matter within a given unit of environmental area.
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Niche
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the role and position a species has in its environment
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Fundamental niche
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The environment that an animal is adapted to naturally
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Realised niche
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The environment that an animal occupies outside of its fundamental niche as a result of lack of resources or habitability
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Primary succession
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An environment where there is life where there was no life before
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In situ
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the natural habitat of an animal
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ex situ
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animals that are not in their natural habitat
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Deliberate release of alien species example
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Savlinia Weevil
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Accidental release of alien species example
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When maori settlers arrived in New Zealand rats where accidentally released from he boats of the settlers.
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Ecological Succession
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Process of change in the species in an environment over time
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Biomagnification
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An increase in concentration of a substance in an animal usually toxic throughout the progression of the food chain
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Gross production
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the amount of chemical energy as biomass that primary producers create in a given length of time
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Net production
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the rate at which all the plants in an ecosystem produce net useful chemical energy
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Steps of the carbon cycle
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1. Carbon enters the atmosphere as carbon dioxide from respiration and combustion 2. CO2 absorbed by producers 3.Primary consumers eat producers 4.Dead consumers and producers decompose in the ground, carbon is retuned to the atmosphere
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Example of biomagnification
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DDT in water to eagles
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What did DDT cause
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Decreased the number of predator birds. The eggs of these birds were easily cracked. In fact, the weight of the mother sitting on the eggs cracked them. It was finally discovered that DDT was building up in the tissue of the birds and interfering with the calcium needed for the shells to be hard
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What units are used when constructing pyramids of energy?
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J m^2 year-1
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Mesocosm
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an experimental tool that brings a small part of the natural environment under controlled conditions.
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Microplastics
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smaller than 5mm, damages digestive systems
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Macroplastics
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greater than 5mm, damages digestive systems
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Endemic species
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Species unique to a geographical area
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What can competitive exclusion affect?
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endemic species
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How to manage the conservation of areas
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Restoration, recovery of threatened species, removal of introduced species, legal protection, funding and prioritising
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what is richness?
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number of organisms
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what is evenness?
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how many of each organism
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what are the two components of biodiversity
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richness and evenness
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biotic index
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the total number of indicator species in a given area
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indicator species
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Species used to measure the conditions of an environment e.g air quality by measuring lichen
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How can general effect of global warming be prevented
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Use fewer fossil fuels, use nuclear power and renewable energy sources such as solar, wind or biofuel, better home insulation, stop deforestation
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Abiotic factors influencing species distribution in communities
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light intensity, atmosphere, water availability, salinity of the soil, soil pH
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Biotic factors influencing species distribution in communitieS
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Other organisms in the ecosystem, inter-relationships between organisms
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Shelfords law of tolerance
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states that the levels of one or more chemical or physical factor determines the abundance and distribution of an organism in an ecosystem
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Define a Keystone species
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A species exhibiting a strong control on the structure of a community
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Give an example of a keystone species
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A sea star pisater
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Spatial habitat
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the area inhabited by an oranges in an ecosystem
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Feeding activity
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What an organism feeds on in an ecosystem to keep the ecosystem in check
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What interactions between species are there?
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Competition, herbivory, predation, parasitism, mutualism
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What is competition?
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When two organisms fight for the same food supply
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What is herbivory?
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primary consumers
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what is predation?
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predators eating consumers
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what is parasitism?
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when an organism lives off of another oragansim
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what is mutualism?
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When two species aid each other in a relationship
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What are the difficulties in classifying organisms into trophic levels
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choosing between secondary and tertiary consumers, omnivores that eat from all trophic levels
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FCR
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measures the efficiency of an organisms ability to take on mass from the food it consumes
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What principles are follow to make sustainable agriculture?
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Maintenance of food, improving the environment, using resources efficiently, improving loves of families and societies
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secondary succession
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when a group of organisms take over a natural primary succession
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Foredune
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No soil, just sand and marram grass
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yellow dune
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thin layer of soil, some animals
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grey dune
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developed layer of humus, further in land
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humus
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organic component of soil from decomposition
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Mature dune
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can support forests, thick humus, wild flowers
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A human interference with nutrient cycling
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overfishing
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Closed ecosystems
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closed systems exchange energy but not matter
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Biological control
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using natural procedures to control unnatural invasive species
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Biosphere
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living organisms on earth
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Biome
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section of the biosphere with distinct characteristics
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Tundra
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high elevation, low temp, low rain
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Coniferous forrest (taiga)
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high elevation, not as cold as tundra, a bit more rain than tundra
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Temperate forrest
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lower elevation, warmer temps, more rain
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Desert
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low elevation, high temperature, no rain
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Tropical forrest
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low elevation, warm temp, moist
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thermal properties of water
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acts as a temperature stabiliser in organisms because it does not change temp quickly, used as coolant in humans during perspiration
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thermal properties of methane
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non-polar, freezing point: -183, boiling point: -163, low energy needed to break bonds, burns
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how were genetic codes used to prove that archea were unique?
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They looked similar to bacteria, and had similar DNA but they didn't have a nucleus, Woese distinguished them from others, they live in harsh conditions
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What did researchers find was the largest cause of Trisomy-21
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age of the mother
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How was it discovered that x-rays were not entirely safe?
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Alice Stewart in the 1950's showed that children whose mothers had prenatal x-rays were twice as likely to die of cancer than children whose mothers didn't
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