Final – Microbiology Answers – Flashcards
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| First Step in Spills Procedure |
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| Notify everyone in room |
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| condenser |
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| Directs light rays onto and through the specimen |
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| Parfocal |
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| Once the object is in focus with lowest power, it should be almost in focus with highest power |
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| Working distance |
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| Distance between the slide and bottom of lens |
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| Resolution |
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| Ability to see details, can be improved with oil |
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| Prepare Bacterial Smear |
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| Sterilize loop Select colony Pick up and mix w/ saline Heat Fix |
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| Gram Stain: Staining |
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| Flood w/ crystal violet for 1 min Flood with iodine for 1 min |
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| Gram Stain: Decolorization |
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| Drip acetone across smear till clear Rinse |
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| Gram Stain: Counter Stain |
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| Flood w/ Safranin for 1 min Rinse Blot dry with bibulous paper |
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| Most Critical Step in Gram Stain Procedure |
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| Rinse acetone |
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| Color after prepared smear for gram stain |
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| gram-=clear gram+=clear |
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| Color after staining in gram stain |
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| gram-=purple gram+=purple |
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| Color after decolorization in gram stain |
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| gram-=clear gram+=purple |
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| Color after counterstain in gram stain |
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| gram-=red gram+=purple |
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| Overcolorize |
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| Crystal violet left on to long Both purple |
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| Overdecolorize |
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| Acetone left on to long Both red |
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| What part of cell determines gram stain reaction |
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| peptidoglycan |
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| Mixed Culture |
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| Presence of more than one bacterial species |
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| 2 Purple Kill-its |
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| kill-it not working |
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| 2 Yellow kill-its |
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| Autoclave not working |
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| Disinfection |
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| Destroy or remove contaminants or microorganisms |
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| Sterelization |
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| Destroys or removes all microorganisms, including endospores and viruses |
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| Viruses most resistant to Decontamination |
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| Poliovirus & Hepatitis B |
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| What tests are used to identify Staphylococcus? |
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| Mannitol Salt Agar Coagulase Test Staphaux Aggluation |
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| Staphylococcus Sp. has what catalase reaction? |
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| positive |
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| Staphylococcus sp. has what gram stain reaction? |
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| positive |
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| Strepylococcus sp. has what catalase reaction? |
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| negative |
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| Strepylococcus has what gram stain reaction? |
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| positive |
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| MIC |
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| minimum inhibitory concentration lowest amount of antibiotic that inhibits growth of an organism |
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| Direct Immunology testing |
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| measures presence of antigen |
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| Indirect immunology testing |
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| Measures presence of antibody |
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| Tests for HIV |
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| ELISA Western Blot-more specific |
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| Blood Agar Plate has 5% of what? |
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| Sheep's Blood |
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| BAP is what kind of plate? |
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| Selective, differential and enriched |
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| Color of Alpha, Beta, and Gamma Hemolysis |
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| alpha-green beta-clear gamma-none |
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| Mannitol Salt Agar turns yellow for what organism |
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| Staphylococcus aureus |
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| Mannitol Salt Salt agar has what indicator |
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| Phenol red pH indicator |
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| MSA grows what gram stains |
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| gram + |
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| MacConkey grows what gram stain |
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| gram - |
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| MacConkey has what indicator |
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| neutral red pH indicator |
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| Lactose fermenters turn what color on MacConkey |
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| pink/red colonies |
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| MacConkey grow what kind of organisms |
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| coliforms |
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| Hektoen grows what kind of organisms and what gram stain |
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| gram - pathogens |
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| Hektoen has what indicator |
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| Bromthymol blue |
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| What organisms grow blue-green colonies on Hektoen? |
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| Salmonella and Shigella |
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| What kind of organisms will turn yellow on Hektoen? |
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| normal flora |
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| Bile Esculin will turn black from what organism? |
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| Enterococcus |
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| Bile esculin grows what gram stain? |
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| gram + |
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| What disease is caused by Clostridium difficille? |
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| Pseudomembranous entercolitis |
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| Neisseria gonorrhoeae has what form and gram stain |
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| gram - diplococcus |
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| Staphylococcus aureus has what catalase, coagulase reaction |
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| catalase + coagulase + |
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| Yeast is what organism and gram stain |
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| Candida albicans, gram + |
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| Simmon's Citrate has what indicator |
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| bromthymol blue |
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| What grows on chocolate media |
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| Neisseria gonorrhea 5% to 10% CO2 |
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| Oxidase positive is what color |
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| purple |
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| Psedemonas aurginosa can produce what color pus |
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| blue-green |
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| What is substrate of catalase test |
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| hydrogen peroxide |
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| Streptococcus pneumoniae has what gram stain reaction and morphology |
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| Gram+ cocci in chains |
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| What disk and what is used in that disk to test for Steptococcus pneumoniae and what type of hemolysis |
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| P-Disk Optochin Alpha hemolytic |
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| Steptococcus pyogenes has what type of hemolysis and what is its full name |
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| Beta Beta-hemolytic Strptococcus pyogenes group A |
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| What disk and what is in that disk to test for strptococcus pyogenes |
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| A-Disk Bacitracin |
