Family Enterobacteriaceae – Flashcards
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Family Enterobacteriaceae Characteristics common to ALL members |
- Gram-negative bacilli or coccobacilli/Rods - Glucose Fermenters - Lactose Fermenters -Capable of reducing nitrate to nitrite -Grow on MAC plates -Motile by peritrichous flagella - Catalase positive -Oxidase negative |
Escherichia coli |
- Motile Gram-Negative bacillus -Lactose Fermenter -Oxidase Negative -Nitrate Positive -Indole Positive -Methyl Red Positive -Voges-Proskauer Negative -Citrate Negative -PYR Negative -MUG Positive -TSI = A/A with or without gas |
Genus Klebsiella |
Nonmotile, Gram-Negative Bacilli Encapsulated, very mucoid colonies (sticky) Lactose Fermenting: MAC (pink); Hektoen (yellow); XLD (yellow) Oxidase Negative Nitrate Positive Indole Negative Methyl Red Negative V-P Positive Citrate Positive MUG Negative TSI = A/AG (abundant gas)
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Genera Pantoea and Enterobacter |
Motile, Gram-Negative bacilli Lactose Fermenting: BAC (smooth); MAC (pink;mucoid?) Hektoen (yellow); XLD (yellow) Oxidase Negative Nitrate Positive Indole Negative Methyl Red Negative V-P Positive Citrate Positive TSI/KIA = A/A usually with abundant gas |
Genus Citrobactor |
Motile, Gram-Negative Bacilli Foul Smelling Late Lactose Fermenter: MAC (light pink); Hektoen (colorless); XLD (red, yellow, or colorless) Indole Variable Methyl Red Positive V-P Negative Citrate Positive TSI/KIA = A/A or A/AG; H2S Positive Oxidase Negative Nitrate Positive Lysine decarboxylase Negative |
Genus Serratia |
Motile, Gram-Negative Bacilli Late Lactose Fermenting: MAC (Red); Hektoen (colorless); XLD (colorless or yellow) Oxidase Negative Nitrate Positive Indole Negative Methyl Red Variable V-P variable Citrate Positive S. marcescens = V-P Positive TSI/KIA; A/A with or without gas |
Genus Salmonella |
Motile, Gram-Negative Bacilli Non-lactose fermenter H2S Producer Produce lysine and ornithine decarboxylase Oxidase Negative Nitrate Positive Urease Negative Indole Negative Methyl Red Positive V-P Negative Citrate Variable TSI/KIA: K/A with or without gas; H2S Positive |
Genus Shigella |
NONMOTILE, Gram-Negative Bacilli Non-lactose Fermenter Oxidase Negative Nitrate Positive Indole Variable Methyl Red Positive V-P Negative Citrate Positive TSI/KIA: K/A with or without gas Urease Negative Lysine decarboxylase Negative |
Genus Proteus |
Motile, Gram-Negative Bacilli Non-lactose-fermenting Oxidase Negative Nitrate Positive TSI/KIA: K/A; H2S Positive PAD Positive Produce Phenylalanine Deaminase
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Proteus mirabilis |
BAP & Chocolate Agar: swarming; MAC (colorless, swarming, foul smelling); Hektoen (colorless); XLD (yellow or colorless)
Indole Negative Maltose Negative Ornithine decarboxylase Positive |
Proteus penneri
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Indole Negative Maltose Positive Ornithine Negative |
Proteus vulgaris |
BAP & Chocolate (swarming); MAC (colorless, foul smelling); Hektoen (colorless); XLD (yellow or colorless)
Indole Positive |
Morganella morganii |
Motile, Gram-Negative Bacillus Non-lactose-fermenting Oxidase Negative Nitrate Positive PAD Positive Indole Positive Methyl Red Positive V-P Negative Citrate Negative TSI/KIA: K/AG; H2S Negative |
Genus Providencia |
Motile, Gram-Negative Bacilli Non-lactose-Fermenting Oxidase Negative Nitrate Positive PAD Positive Indole Positive Methyl Red Positive V-P Negative Citrate Positive TSI/KIA: K/A; H2S Negative |
Genus Yersinia |
Nonmotile, Gram-Negative Bacillus Non-lactose-fermenting CATALASE POSITIVE Oxidase Negative Nitrate Positive Urease Negative Indole Negative Methyl Red Positive V-P Negative Citrate Negative Esculin Positive TSI/KIA: K/AG Ornithine decarboxylase Negative Ferments glucose without producing Gas |
