Exam 1 – Microbiology Flashcard

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organism
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any living thing

 

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pathogen
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disease causing organism
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pathogenicity
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ability of pathogen to gain entry to host tissue and bring about disease
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virulence
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"full of poison"

 

DEGREE to which a pathogen causes disease

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Prokaryotic
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a cells whose genetic material is not enclosed in a nuclear membrane
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Eukaryotic
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a cell having DNA inside a distinct membrane-enclosed nucleus

 

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Binomial Nomenclature species
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the system of having two names

 

(Clostridium tetani)

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Genus
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a group of closely related species

 

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sp
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refers to a species in singular-

 

uknown species of a known genus

 

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spp

 

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refers to a species in plural

 

refers to all members of a genus

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Strain
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indentified by numbers, letters or names that follow species

 

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Clone
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group of organisms all derived from a single parent cell
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Cocci
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Single Cell

 

(circle form)

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Bacilli
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Rod shaped
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Spirilla
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sprial shape
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Bacterial capsule
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the glycocalyx is organized and formly attached tothe cell wall forming thick layer outside of the cell
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Flagella
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Long whip like structure for motility
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Axial filaments
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spirochetes move by means of axial filaments
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fimbriae
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short, straight projections for attachment involved in biofilm formation that allow attachment to a host
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sex Pilus
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longer, one--two per cell for transfer of the DNA in mating process

 

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Endospore
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a survival form of bacteria produced within the cell in response to adverse environmental conditions
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Pathology
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Etiology
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Study of cause of disease
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Infection
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state were host harbors microbes that survive and multiply in body tissue
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Disease
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process or even that results in any change from the general state of health
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Opportunistic pathogens
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Acute disease
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develops rapidly and last a short period of time
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Chronic disease
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develops slowly, lasting a long time
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Communicable disease
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noncommunicable disease
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primary infection
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1st pathogen to infect a body part
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secondary infection
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infection of a body part by a 2nd pathogen
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Compromised host
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Zoonoses
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a disease of animals that can be transmitted to humans
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Fomite
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Vectors

 

(mechanical and biological)

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Epidemiology
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the study when and where diseases occur and how they are transmitted and occurence
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Adhesins
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Phagocytosis
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Collagenase
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dissolves collagen; softens tissue so it can spread
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Leukocidin
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kills white blood cells; decreases hosts resistance
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Hemolysin
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causes hemolysis

(lysis of red blood cell)

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Hemolysis:

 

alpha

beta

gama

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alpha--partial : greenish zone around colony

 

Beta--complete hemolysis--clear, odorless zone around colony

 

Gama- No hemolysis

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5 baisc groups of Microbes
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  • Fungi
  • Protozoa
  • Virsuses
  • bacteria
  • algae
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beneficial effects of microbes
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  1. oxygen (from the ocean 50%)
  2. food
  3. soil microbes break down waste
  4. microbes in intestines for digestion
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Anton van Leeuwenhoek
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  • 1st person to observe microbes through magnifying glass
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Ernst Ruska
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made first Electron microscope
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Ignaz Semmelweiss
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hospitals needed to wash hands
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Louis Pasteur
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Germ theory of disease
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Robert Koch
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first to  cultivate anthrax
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Microbial Taxonomy
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classification of microorganisms
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Virulence degrees (3)
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  1. Highly virulent
  2. moderatly virulent
  3. avirulent
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Prokaryotic and Eukaryotic similarities
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  • both have membranes
  • both use DNA to encode genetic material
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What do prokaryotic cells lack?
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internal membrane-enclosed organelles
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Characteristics of Prokaryotes
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  • unicellular
  • contains one circular chromosome
  • no nucleolus
  • lacks membrane-enclosed organelle

 

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Cell wall of Prokaryotes
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  • Contains polyssacharide peptidoglycan
  • has flagella
  • No cilia
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Cell wall of Eukaryotes
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  • cell wall is chemically simple
  • may have cilia or flagella
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Binary fission
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[image]
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mitochondria and chloroplast have how many ribosomes?
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70s
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what does the THREE DOMAINS consist of?
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  • two prokaryotes
  • one eukaryotes
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What are the THREE DOMAINS?
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  1. Archaea
  2. Bacteria
  3. Eukarya
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Classifications of THREE DOMAINS?
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  1. Archaea--live in extreme habitates, bacteria-like
  2. Bacteria--cell wall have peptidoglycan
  3. eukarya--all ekaryotic organisms
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Bacteria are:
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  • Prokaryotic
  • have cell wall
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Shape of coccus?
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Spherical

 

pairs=diplococci

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name of cocci in 4s?
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tetrads
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name of cocci in 8s?
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sarcinae
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Streptococci shape
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chaines
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shapes of staphylococci
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grape-like
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Vibrios
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curved like rods
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prirochetes
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long spirilla with many tight coils
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Glycocalyx
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substance that surrounds cell
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what is the glycocalyx made of?
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Slimey layer
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Function of Bacterial Capsule
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  • enables bacteria to resist phagocytosis by host defense cells
  • allows some bacteria to adhere to surfaces and colonize and resist flushing
  • prevents cell from dehydration and chemicals and loss of nutrients
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Biofilm
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a microbial community that forms a slimy layer on a surface
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CDC says biofilms are involved in what percentage of all human bacterial infection?
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70%
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Function of biofilm
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  • Resist disinfectants
  • resist anti-biotics
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Bacteria that lack flagella are called?
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atrichous
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4 types of arrangements of flagella
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  1. monotrichous- single
  2. amphitrichous-single and both ends
  3. lophotrichous-two or more at one or both ends
  4. peritrichous- all over cell

 

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Gram positive characteristics
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  • layers of peptidoglycan
  • teichoic acids= alcohol and phosphate (function as antigen)
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why is it important to know gram + from gram - ?

 

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to choose the best control method
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characteristics of Negative cell wall
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  • one or few peptidoglycan and outer membrane of protein and fat
  • no teichoic acid
  • lipplyssacharide in outter membrane
  • lipoproteins and phospholipids too
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primary stain color?
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crystal violet
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mordant define
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agent that fixes the stain to cell
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if iodine present in gram staining?
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cells become darker violet
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countersaint name and color?
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  • Safranin
  • turn reddish-pink or remain purple
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Lipid bilayer
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the phospholipids that are arranged in two parallel rows
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cytoplasm
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cell liquid
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2 subunits of ribosomes
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  1. protein
  2. ribosomal RNA
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Plasmid
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  • bacteria that contain circular, double-stranded DNA
  • small loops of extrachromosomal DNA
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Function of plasmid
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  1. carry genes for virulence or drug resistence
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Sporulation
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process of endospore formation within a vegitative cell
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germination
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endospore returns to its vegitative state
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how to kill spores
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  • steam autoclave
  • gas autoclave
  • some disinfectants
  • powerful radiation
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Symbiosis
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relationship between normal flora and host
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Opportunistic microbes
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  1. require a special opportunity to produce disease such as injury or impaired host defenses
  2. same organism can often produce a variety of problems
  3. common organisms, frequently encounteredor already carried
  4. cause secondary infection
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True pathogens
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  1. able to infect healthy host under own power and initiate disease
  2. produces one specific disease
  3. not so common
  4. cause of primary infection
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What did Koch Postulate?

 

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  1. organism must be present in every case of disease
  2. organism must be isolated in pur culture from a diseased host
  3. pure culture must produce the disease when inoculated into a healthy, susceptible host
  4. organism much be re-isolated in pure culture from infected experimented host
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Function of Kochs postulation
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  • to prove that certain microbes cause certain diseases
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endemic
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  • constantly present in a population
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subacute disease
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disease that is intermediate between acute and chronic
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Focal Infection
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  • local infection enters a blood vessel and spreads to other parts of body
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Bacteremia
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bacteria in blood
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Sepsis
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  • toxic inflammatory condition arising from the spread of microbes and toxins
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what does sepsis cause?
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  • edema
  • dyspnea (breathing diff)
  • fever
  • confusion
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once in body, what are the sequence that disease follows?
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  1. incubation period
  2. prodromal period
  3. period of illness
  4. period of convalenscence
  5. contagious period
  6. levels of recovery

 

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Vectors
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an organism that carries a pathogen such as animals carry pathogens from on host to another
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Noscomial infection
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infection aquired in hospital
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Morbidity
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incidence of a specific disease
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exotoxins
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  • produced inside some bacteria then secreted outside
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Release of EXOTOXIN from LIVING bacterium
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Release of ENDOTOXIN from DEAD bacterium
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M protein
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found on cell surface and fimbriae
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