Culture Media – Flashcards
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| EMB |
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| Eosin Methlylene Blue, selects for G- and lactose fermentation |
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| PEA |
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| Phenylethyl alcohol selects for G+ |
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| MacConkey |
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| Crystal violet and bile salts select for G-, lactose fermentation is detected by Neutral Red pH indicator |
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| Starch |
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| Use Iodine as the reagent to detect amalyase activity |
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| Litmus Milk |
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| Fermentation of lactose, use of casein protein (proteolysis). Litmus is the pH indicator (pink-blue), and redox indicator (white=reduced) |
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| Triple Sugar Iron |
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| Sugars flucose, sucrose, and lactose, pH is phenol red (Y-R) gases CO2 and H2H may be produced use FES as indicator. |
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| OF medium |
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| Fermentation of glucose will turn medium yellow (from thymol blue Y-B), indicates fermentation or oxidation |
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| BGA |
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| Food micro for detection of Gram- lactose fermenters selective agent and indicator is brilliant green dye (Green-Red) |
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| m-Endo |
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| Selective and differential, basic fushsin selects for G- lactose and sodium silfite and dye differentiate. |
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| Phenol Red |
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| pH indicator yellow as acid |
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| OF Agar Indicator |
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| Bromthymol Blue |
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| Brilliant Green Agar Differential Agent |
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| Lactose |
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| Most Probable Number (MPN) |
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| Must do conformation and usually for water purity |
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| Gelatin |
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| shows the presence of gelatinase if there is liquification |
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| IMViC |
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| indole, methyl red, voges-proskauer, citrate |
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| Indole |
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| shows pressence of tryptophanase pink ring on top (E. Aerogenes does not have) |
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| Methyl Red |
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| Yellow Postive (E. Coli) negative (E.aerogenes) |
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| Voges-Proskauer |
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| E. aerogenes is negative neutral products/ pink is a positive |
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| Citrate |
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| pH indicator is brom-thymol blue (yellow-blue)- sodium citrate as sole carbon source |
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| Urea |
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| Urease, positive is red |
