Chromatography: Separation by Adsorption

A separation technique in which a mobile phase carrying a mixture moves in contact with a selectively *adsorbent* stationary phase

The separation of a mixture of components occurs as a result of selective adsorbance of the components of the mixture on a stationary phase while carried out by a mobile phase

Use gloves, oil from skin can affect results

You may use paper clip and glass rod instead, pour water to depth of about 1cm if water soluble, use an organic solvent if they are 'water insoluble', mark a line on the paper with pencil of where the original sample of marker is put, making sure they are above solvent level

Solvent rises up though paper, separation of colour

The solvent is the mobile phase and as it moves up through the stationary phase (chromatography paper), the components of the mixture are adsorbed to a different extent on the paper

Change the solvent/use a mixture of solvents, it will improve or worsen based on the nature of the solvent

Which inks are mixtures of other inks and the inks they're a mixture of

Universal indicator or chlorophyll or alcohol levels in blood

Apply mixture using dropper on paper about 2cm above solvent, place in tank with solvent, solvent moves up or down, state separation of components of mixture

Different absorbance on mobile and stationary phases