Gene Splicing Lab
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Plasmid
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A small, circular section of extra DNA that confers one or more traits to a bacterium and can be reproduced separately from the main bacterial genetic code.
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pAMP
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Plasmid that confers resistance to ampicillin
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pKAN
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Plasmid that confers resistance to kanamycin
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Ligation
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A reaction to link two separate DNA molecules via the formation of a phosphodiester bond between the 3'-hydroxyl end of one and the 5'-phosphate of the other
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Restriction Enzyme
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Cut DNA into shorter fragments in a predictable and controllable manner. Each recognizes a specific 4 to 6 base-pair nucleotide sequence
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Restriction Fragment
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the length of a restriction fragment, A DNA segment that results from the cutting of DNA by a restriction enzyme.
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Sticky ends
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Fragments of DNA with exposed complimentary nucleotides at the end that allow them to be attached to other DNA fragments
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Suspension
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A mixture in which particles can be seen and easily separated by settling or filtration
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Drosophila Melanogaster
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fruit fly. Useful for biological experiments b/c it has a very low chromosome number.
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Female Drosophila
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Larger, alternating light and dark bands on rear of abdomen, ovipositor plates, no sex comb
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Male Drosophila
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Smaller, uniform pigmentation, genital arch and penis, sex comb.
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Apterous
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wingless
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Vestigial
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remnant of a structure that may have had an important function in a species' ancestors, but has no clear function in the modern species.
