Microbiology-Chapter 4 – Flashcards

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question
Which of the following is NOT found in all bacterial cellsa. cell membrane
b. nucleoid
c. ribosomes
d. actin cytoskeleton
answer
d. actin cytoskeleton
question
Pili are tubular shafts in ______bacteria that serve as a means of _____________.
a. gram-positive; genetic exchange
b. gram positive; attachment
c. gram negative, genetic exchange
d. gram-negative; protection
answer
c. gram negative, genetic exchange
question
An example of a of a glycocalyx is:
a. a capsule
b. a pilus
c. an outer membrane
d. a cell wall
answer
a. a capsule
question
Which of the following is a primary bacterial cell wall funtiona. transport
b. motility
c. support
d. adhesion
answer
c. support
question
which of the following is present in both gram-positive and gram-negative cell wallsa. outer membrane
b.peptidoglycan
c. teichoic acid
d. lipopolysaccharides
answer
b.peptidoglycan
question
Dark stained granulues are concentrated crystals of ____that are found in ______.
a. fat, myobacterium
b. dipicolinic acid, Bacillus
c. sulfur, Thiobacillus
d. PO4, Corynebacterium
answer
d. PO4, Corynebacterium
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Bacterial endospores usually function in
a. reproduction
b. survival
c. protein synthesis
d. storage
answer
b. survival
question
A bacterial arrangement in packets of eight cells is described as a ______.
a. micrococcus
b. diplococcus
c. tetrad
d. sarcina
answer
d. sarcina
question
To which division of bacteria do cyanobacteria belonga. Tenericutes
b.Gracilicutes
c.Firmicutes
d. mendosicutes
answer
b.Gracilicutes
question
Which stain is used to distinguish differences between the cell walls of medically important bacteriaa. simple strain
b. acridine orange stain
c. gram stain
d. negative stain
answer
c. gram stain
question
True or False
one major difference in the envelope structure between gram-Positive and gram-negative bacteria is the presence or absence of a cytoplasmic membrane
answer
False: one major difference in the envelope structure between gram-positive bacteria and gram-negative bacteria is the presence or absence of an outer membrane
question
True or False
A research microbiologist looking at at evolutionary relatedness between two bacterial species is more likely to use Bergey's Manual of Determinative Bacteriology than Bergey's Manual of Systematic Bacteriology
answer
False: A research microbiologist looking at evolutionary relatedness between two bacterial species is more likely to use Bergery's manual of systematic bacteriology
question
True or False
Nanobes may or may not actually be bacteria
answer
True
question
True or False
Both bacteria and archaea are prokaryotes
answer
True
question
True or False
a collection of bacteria that share an overall similar pattern of traits is called a species
answer
True
question
Name the structures that all bacteria possess
answer
EXTERNAL:
appendages :flagella
pili
fimbriae
Glycocalyx (capsule-slime layer)
Cell envelope (outer membrane)
-cell wall
-cell membrane
Internal-- cytoplasm; ribsomes; inclusions; nucleoid/chromosome; actin cytoskeleton; endospore
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cell wall
answer
a semirigid casing that provides structural support and shape for the cell
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cell (cytoplasmic) membrane
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A thin sheet of lipid and protein that surrounds the cytoplasm and controls the flow of materials into and out of the cell pool
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Outer membrane
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extra membrane is similar to cellmembrane but also contain lipopolysaccharide. Controls the flow of materials and portions of it are toxic to mammals when released.
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cytoplasm
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water based solution filling the entire cell
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inclusion/granule
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stored nutrients such as fat, phosphate, or glycogen deposited in dense crystals or particles that can be tapped into when needed
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Plasmid
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Double-stranded DNA circle containing extra genes
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Bacterial chromosome or nucleoid
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composed of condensed DNA molecules. DNA directs all genetics and heredity of the cell and codes for all proteins
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Plasmid
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Double-stranded DNA circle containing extra genes
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Ribosomes
answer
Tiny particles composed of protein and RNA that are the sites of protein synthesis
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Actin Cytoskeleton
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Long fibers of proteins that encircle the cell just inside the cell membrane and contribute to the shape of the cell.
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Endospore
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Dormant body formed within some bacteria that allows for their survival in adverse conditions
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Glycocalyx
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Pink coating - a coating or layer of molecules external to the cell wall. It serves protective, adhesive, and receptor functions
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Pilus
answer
An elongate, hollow appendage used in transfers of DNA to other cells.
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Fimbriae
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fine hairlike Bristles, extending from the cell surface that help in adhesion to other cells and surface
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flagellum
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specialized appendage attached to the cell by a basal body that holds a long rotating filament. The movement pushes the cell forward and provides motility
question
Flagellum:
3 parts
and describe basal wall.
answer
3 parts: filament, Hook (sheath) and Basal body- the basal body is anchored through the cell wall and outer membrane. Hook and filaments rotate 360
question
How do prokaryotes differ from Eukaryotes?
answer
Proks-
1 chromosome
No histones
smaller than X10
all are microbes
bacteria have peptido/Gly can cell walls

Euks-
Nucleus- chromosome 1 or more
bundled by histone proteins

Organnelle-mitochondria nucleus
some are microbes-fungi; algae; protoza; helminths
question
What are examples of disease caused by prokaryotes?
answer
strep throat, lymes disease and ear infections
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Examples of Eukaryotic infections?
answer
histoplasmosis and malaria
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Specific structures that are found in some but not all prokaryotes?
answer
flagella; pili; fimbriae; inclusions, endospores, and intracellular membranes
question
Name at least four structures that some, but not all, bacteria possess.
answer
-Ribsome-
-cell membrane-
-nucleoid
-chromosome
question
Ribsome
answer
-Ribsome-tiny particle composed of protein and RNA that are the sites of protein synthesis
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Cell membrane
answer
-cell membrane- a thin sheet of lipid and protein that surrounds the cytoplasm and controls the flow of materials into and out of the cell pool
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-nucleoid
answer
the basophilic nuclear region or nuclear body that contains bacterial chromosome
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-chromosome
answer
Tightly coiled bodies in cells that are the primary sitesof genes
question
Name the 2 general patterns of flagella
answer
1. polar arrangement-flagella are attached at one or both ends of the cell
A. Monotrichous
B. lophotrichous
c. amphitrichous

2. peritrichous
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peritrichous flagella
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flagella are dispersed randomly over the surface of the cell
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monotrichous
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single flagellum
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lophotrichous
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small bunch or tuffs of flagella emerging from the same site
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amphitrichous
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flagella at both poles of the cell
question
Describe the polar movement of the flagellum?
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a polar flagellum rotating counterclockwise moves the cell forward. Reversing the direction causes the cell to stop and tumble.
question
Describe the movement of flagella rotation
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flagella rotation due to proton gradient. Protons are made from bacterial metabolism
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Describe how peritrichous move:
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Flagella sweep toward one end of the cell and rotate as a group.
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chemotaxis
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flagellar can detect and move in response to chemical signals
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Explain how flagellum works in the presence of an attractant?
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positive chemotaxis is movement of a cell in the direction of a favorable chemical stimulus(usually a nutrient); negative chemotaxis is movement away from a repellant (potientally harful) compound
question
periplasmic flagella- corkscrew shaped bacteria called (a)how do they move (b)
Location (c) and an example (d)
answer
(a) spirochetes
(b) causing a twisting flexing motion "wiggle"
(c) located between the cell wall and cell membrane
(d) Example: spirochetes; lyme, sphillis
question
Pili are found only on: (a) and are involved in conjugation, form cytoplasmic connections between bacteria
answer
Gram negative bacteria
question
Glycocalyx-
Describe
function
outside layer can be?
answer
Bacterial surface coating of repeating polysaccharide units, protein or both.
Function: protects cell and helps it adhere to enviroment
Outside layer can be Slime (loosely bound) or Capsule (tightly bound to cell)
live in damp area
question
Specialized function of glycocalyx?
answer
capsules are formed by pathogenic bacteria Streptococcus pneumonia (cause of pneumonis) Haemophius influenzae (causes mengitis) the capsule protect the bacteria against white blood cells, the bodies natural defense against infection.
question
How can you tell that a bacteria has a large capsule on a petri plate?
answer
Raised and shiny
question
Describe the 3 major shapes of prokaryotes?
answer
Coccus (round)
Bacillus (rod)coccobacillus-short and plump; vibrio -gently curved

spiral (2types spirochetes and spirilla)
question
List some of the difference between archaea and bacteria?
answer
Cell structure
metabolism
genetics
question
Difference between archaea and bacteria?
answer
found in extreme enviroments-
another type of prokaryotic cells that constitiute the third domain of life. They exhibit ususual biochemistry and genetic make up. They adapt to extreme habitats with low or high temperature, salt, pressure, or acid
question
Explain what a species is?
answer
is a collection of bacterial cells, all of which share an overall an overall similar pattern of traits
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