Biology quiz 4 – Flashcards
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A research team began a study of a cultured cell line. Their preliminary observations showed them that the cell line did not exhibit either density-dependent inhibition or anchorage dependence. What could they conclude right away?
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The cells show characteristics of tumors.
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Through a microscope, you can see a cell plate beginning to develop across the middle of a cell and nuclei forming on either side of the cell plate. This cell is most likely
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a plant cell in the process of cytokinesis.
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Which of the following is a protein maintained at constant levels throughout the cell cycle that requires cyclin to become catalytically active?
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cdk
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The decline of MPF activity at the end of mitosis is due to
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the degradation of cyclin.
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Suppose a biologist can separate one of a dozen pieces of chromatin from a eukaryotic (animal) nucleus. It might consist of which of the following?
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two long strands of DNA plus proteins
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Which of the following describe(s) cyclin-dependent kinase (Cdk)?
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Cdk is present throughout the cell cycle and is an enzyme that attaches phosphate groups to other proteins.
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Which of the following was a discovery that had to be made before human chromosomes could be correctly counted?
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how to use a hypotonic solution to swell nuclei
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After which checkpoint is the cell first committed to continue the cell cycle through M?
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G1
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Which of the following triggers the cell's passage past the G2 checkpoint into mitosis?
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MPF
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The MPF protein complex turns itself off by
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activating a process that destroys cyclin components.
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A plant-derived protein known as colchicine can be used to poison cells by blocking the formation of the spindle. Which of the following would result if colchicine is added to a sample of cells in G2?
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The chromosomes would coil and shorten but have no spindle to which to attach.
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The cyclin component of MPF is destroyed toward the end of which phase?
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M
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Which of the following does not occur during mitosis?
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replication of the DNA
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Why do chromosomes coil during mitosis?
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to allow the chromosomes to move without becoming entangled and breaking
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Where do the microtubules of the spindle originate during mitosis in both plant and animal cells?
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centrosome
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Which of the following is a protein synthesized at specific times during the cell cycle that associates with a kinase to form a catalytically active complex?
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cyclin
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Which of the following are primarily responsible for cytokinesis in plant cells but not in animal cells?
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golgi-derived vesicles
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For a chemotherapeutic drug to be useful for treating cancer cells, which of the following is most desirable?
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It interferes with rapidly dividing cells.
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If there are 20 chromatids in a cell, how many centromeres are there?
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10
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If there are 20 centromeres in a cell at anaphase, how many chromosomes are there in each daughter cell following cytokinesis?
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10
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Which of the following is true concerning cancer cells?
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When they stop dividing, they do so at random points in the cell cycle; they are not subject to cell cycle controls; and they do not exhibit density-dependent inhibition when growing in culture.
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Besides the ability of some cancer cells to overproliferate, what else could logically result in a tumor?
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lack of appropriate cell death.
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What causes the decrease in the amount of cyclin at a specific point in the cell cycle?
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its destruction by a process initiated by the activity of its complex with a cyclin
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Proteins that are involved in the regulation of the cell cycle, and that show fluctuations in concentration during the cell cycle, are called
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cyclins
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If cells in the process of dividing are subjected to colchicine, a drug that interferes with the formation of the spindle apparatus, at which stage will mitosis be arrested?
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metaphase
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A student is looking through his light microscope (~450 X) at a squashed and stained onion root tip. Some, but not all, of the cells have clearly visible chromosome strands. what does he see?
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two small cells with chromatin
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Density-dependent inhibition is explained by which of the following?
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As cells become more numerous, the cell surface proteins of one cell contact the adjoining cells and they
stop dividing.
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Which of the following most accurately describes a cyclin?
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It activates a Cdk molecule when it is in sufficient concentration.
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Which of the following describe(s) cyclin-dependent kinase (Cdk)?
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Cdk is present throughout the cell cycle and is an enzyme that attaches phosphate groups to other proteins.