MMB Ch.6 – Flashcards

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A cell that uses an organic carbon source and obtains energy from light would be called a
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Photoheterotroph
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Prokaryotes living in the ocean around volcanic vents use the volcanic gasses carbon dioxide (CO2) and hydrogen sulfide (H2S) to fuel their metabolism. They are therefore
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Lithoautotrophs
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A microbe recovered from an environmental sample grows on a medium composed of agar and a mix of salts. The agar is not digested during incubation. The growth rate increases in high CO2 conditions. The organism is likely a(n)
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Autotroph
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An aquatic microbe that can grow only near the surface of the water is probably which of the following?
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Phototroph
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An organism that produces catalase enzyme is protected from the harmful effects of
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Peroxide anion
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Which of the following oxidizing agents is sometimes produced during aerobic respiration?
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Superoxide radical
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A microbe that grows only at the bottom of a tube of thioglycollate medium is probably a(n)
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Obligate anaerobe
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A food product is labeled as having antioxidants. It may therefore contain
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Vitamin C, or E, or both
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An organism that requires chemical nutrients which serve as final electron acceptors is a(n)
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Chemotroph
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At temperatures higher than the maximum growth temperature for an organism,
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hydrogen bonds are broken, proteins are denatured, and membranes become too fluid.
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The ________ are an important source of nitrogen compounds that can be metabolized by most other organisms.
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Cyanobacteria
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You open a jar that has been in the refrigerator for a couple of weeks and find the contents do not look or smell right. What type of microbe has been growing in your refrigerator?
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Psychrophile
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Bacteria living in communities of microbes change the genes they are expressing in response to
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Quorum-sensing molecules
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Organisms that can grow with or without oxygen present are
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either facultative anaerobes or aerotolerant anaerobes.
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A fastidious organism might be grown on which of the following types of media
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Enriched media
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Obligate anaerobes may be cultured in the laboratory
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In a reducing medium
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Joan wants to discover a microbe capable of degrading an environmental contaminant. Which of the following is the process she should use
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Enrichment culture
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microbiologist inoculates a growth medium with 100 bacterial cells/ml. If the generation time of the species is 1 hour, and there is no lag phase, how long will it be before the culture contains more than 10,000 cells/ml?
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7 hours
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An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be success
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Inoculation of cell cultures
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Microbial growth rates are controlled in a chemostat by
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Controlling the amount of limiting nutrient
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MacConkey agar plates represent ________ medium
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Both a differential and a selective
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A Petroff-Hauser counting chamber is a(n)
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glass slide containing an etched grid for counting microbes directly using a microscope
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The best method for preserving bacterial cells in storage for decades
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Lyophilization
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A device that removes wastes and adds fresh medium to bacterial cultures in order to prolong the log phase of a culture is called
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Chemostat
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Bacterial binary fission results in ________ growth
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Exponential
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Which of the following is an indirect method for estimating the number of microbes in a sample
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Turbidity
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During which growth phase do antimicrobial drugs have the greatest inhibitory effect
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Log phase
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Which of the following explains why honey can be stored at room temperature for years without spoiling
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Osmotic pressure
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All of the following are part of the process of binary fission EXCEPT
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Budding
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The method of obtaining isolated cultures that utilizes surface area to physically dilute specimens is called
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The steak-plate technique
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