Microscope Test – Flashcards

Robert Hooke

Zacharias and Hans Jansen

Ernst Ruska

Anton van Leeuwenhoek

There are several purposes of the lenses in a compound light microscope they are to bend light and make objects appear larger than they really are. Light microscopes today are more modern , so they can magnify objects up to 1,000 times the size.

An important advantage of compound light microscopes are that scientists can observe living cells with them.

Two types of electron microscopes are the scanning electron microscope (a.k.a SEM) and the transmission electron microscope (a.ka. TEM) The advantage of using electron microscopes over light microscopes is that they can magnify objects up to a million times. Also electron microscopes can produce images of much smaller objects than light microscopes can. The disadvantage of using an electron microscope over a light microscope is that they cannot be used to study live specimens.

The major difference between the SEM and the TEAM is in the SEM you can only see the exterior of the cell, while in the TEM you can see what is in the inside of the cell. By looking at the interior of the cell it may give more detail, and description than just looking at the exterior of the cell.

Definition - A microscope that magnifies small objects so that they can be seen easily by using two or more lenses tiny particles called electrons are used to produce magnified images clearer and more detailed than the Light Microscopes living things cannot be viewed in this microscope because the preparation process kills them don't use light Two types (SEM) and (TEM) Transmission Electron Microscope ( TEM)- electrons pass through the specimen and produce a flat image Scanning Electron Microscope (SEM)- electrons bounce off the surface of the specimen and produce a 3D image

Definition - An instrument that magnifies small objects so that they can be seen easily by using two or more lenses three main parts : a tube with two lensed , a stage, and light. May be colored to be more visible items are placed on stage so that the light can be seen through the lenses at the ends of the tube magnify the image light passes through the specimen and produces a flat image ocular lens is what you look through objective lenses re the small lenses that go over the specimen that you can change

1 . Use lens paper to clean the glass slide and a coverslip 2 . Place the specimen hat you wish to observe in the center of the slide 3 . Using a dropper , place one drop of water on the specimen 4 . Hold the slip at the edge of the water and at a 45 degree angle to the slide. 5 . Lower the coverslip slowly to avoid trapping air bubbles 6 . Add more water if needed to keep specimen fresh. Remove access water from slide my using a towel as a blotter. Don't lift the cover slip when doing this

100x

400x

40x

1- body tube 2- rotating eyepiece 3- scanning obj. 4- low power obj. 5- high power obj. 6- stage clips 7- diaphram 8- light 9- eyepiece 10-arm 11- stage 12- coarse focus 13- fine focus 14- base