Micro-Organism – Flashcards
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Unlock answers| Staphlococcus aureus: gram + or gram - |
| gram positive |
| Escherichia coli: gram + or gram - |
| gram negative |
| Salmonella typhimurium : gram + or gram - |
| gram - Enterobacteriaceae |
Characteristic: LOS is readily shed from the bacteria as they grow |
| Neisseria sp. |
| Gram-positive soil bacteria which form a highly resistant form of cells to permit them to live through harsh conditions: endospores? |
| Bacillus and Clostridium |
| E. Coli |
| Facultative anaerobe |
| Which bacterial use the Entner-Doudoroff Pathway instead of glycolysis. |
Pseudomonas (gram negative rod) and Enterococcus faecalis (gram positive rod) |
| Bacteria (2) which are products of Fermentation. |
| Steptococcus and Lactobacillus produce lactic acid |
| Bacteria (2) which are enteric. |
| E. Coli and Salmonella |
| Bacteria which produce H2, CO2, acetone, ethanol, butyrica acid, butanol, and isopropanol |
| Clostridium |
| Bacteria which produce CO2, propionic acid? |
| Propionibacterium |
The most virulent bacteria found in the upper respiratory tract and skin of all warm-blooded animals?
Medically important |
| S. aureus |
| Auramine O is used to detect this bacteria. |
| Mycobacteria tuberculosis |
| Indian Ink (negative staining) outlies the thick carbohydrate capsule characteristic of this yeast. |
| Cryptococcus neoformins |
| Acid fast is used to stain cells of this genus. |
| Mycobacterium and Nocardia and oocysts of certain protozoa |
| Metachromatic staining of volutin is used to detect this bacteria. |
| Corynebacterium diphtheriae |
| Bacteria grown on skim milk agar to promote development of the capsule of this bacteria. |
| Klebsiella pneumoniae |
| Chocolate Agar is use to culture this species. |
| Neisseria and Haemophilus |
| Lack catalase? |
| Streptococcus, Enterococcus, Clostridium |
| Catalase test can be used to distinguish which bacteria? |
Staphylococcus from Streptococcus AND Bacillus from Clostridium
|
| Important oxidase positive gram negative genera (2). |
| Pseudomonas and Neisseria |
| Coagulase test is important for identification of virulence. Which bacteria is the ost virulent> |
| Staphylococcus |
| Group A strep suspected in step throat |
| Streptococcus pyogenes |
| Sterotyping (use of antibodies) to detect O antigen of LPS |
| E.coli stereotype O157 |
| Used to show patterns of susceptibility to antibiotics? |
Bacitracin susceptibility of Group A step
Novobiocine resistance of Staphyococcs saphrophyticus |
| 2 important catalase negative, gram-positive bacteria? |
| Streptococcus and Enterococcus |
| Catalase positive bacteria |
| Staphylococcus spp. |
Catalase test is used to tell large rods apart, which are they? |
Bacillus (catalase positive) AND Costridium (catalase negative) |
| Test which are used clinically and FDA approved to screen for STD's? |
Neisseria gonorrhoeae and Chlamydia trachomatis mycobacteria group A Streptococcus |
| Cells in clusters and catalase (+) |
| Staphyococcus |
| Cells in pairs or chains and catalase (-) |
| Streptococcus |
| Name gram-positive aerobic bacteria (3)? |
Corynebacterium Bacillus Listeria |
| Name gram-positive anaerobic rods? |
Clostridim (spores) ; Actinomyces (branching) |
| Staphylococcus coagulase (+) |
| S. aureus |
| Staphylococcus coagulase (-) |
S. epidermidis S. saphrophyticus |
| Three categories of Streptococcus |
beta hemolytic alpha-hemoytic non-hemolytic |
| Sub-groups of beta-hemolytic |
Group A (bacitracin S; Strep. pyogenes) Group B (CAMP test) Group C Group D (enterococci) Group G ; |
| Sub-groups of alpha-hemolytic |
Strep. pneumoniae (optochin S.) Viridans strep. (optochin R) |
| Sub-groups of non-hemolytic |
anerobic strep (Peptostreptococcus) some enterococci |
| Stain poorly or not at all with Gram stain and are gram positive |
Mycoplasm (no cell wall) Mycobacterium (acid fast) |
Stain poorly or not at all with Gram ; Borrellanegative cell wall structure |
Spirochetes: Treponema Lapospira ; AND ; Rickettsia, Coxiella, Chlamydia (intracellular bacteria) |
Simple growth requirements of gram-negative, aerobic, rods |
lactose fermenters lactose non-fermenters others |
| Name 2 lacotose fermenters |
| Escherichia and Keibsella (mucoid) |
| Name 4 lactose non-fermenters |
Salmonella Shigella Proteus (urease +) Serratin (DNase +) ; |
| Other gram-negative, aerobic, rods with simple growth requirements |
Pseudomionas oxidase (+) Vibrio (comma shape) |
| Gram-negative aerobic, rods with fastidious growth requirements |
Legionella (charcoal yeast agar) Haemophilius (X and Y factors) Bordatella (Bordet-Gengou agar) Campylobacter (seagull shape, 43 oci) |
| Gram-negative anerobic, rods |
Fusobacterium Bacteriodes |
| Gram negative Cocci are defined as |
| cells in pairs and oxidase (+ |
| Examples of oxidase (+) gram-negative cocci |
Neisseria Moraxella |
| Pasteurize to Remove pathogens name 3 examples |
Mycobacterium bovis Coxiela burnetii ;spoilage organisms |
| Relative Susceptibility of Microorganism from most susceptible to most resistant |
Enveloped virses Gram-positive bacteria (most susceptible) Nonenveloped viruses Fungi Gram-negative Active stage protozoa (trophozoites) Cysts of protozoa Mycobacteria Bacterial endospores (most resistant) ; ; ; |
| What is the name of the commerical endospore used to test if an autoclave is working properly.; |
| Bacillus stearothermophilus |
| Use to measure the effectiveness of dry heat sterilization |
| Bacillus subtilis (resistant to killing by dry air in contrast to B. Stearothermophilus) |
| Amphyl is a disinfectant commonly used in biology and microbiology laboratories and effective against |
Pseudomonas aeruginosa Staphylococcus aureus Salmonella choleraesusis Mycobacterium tuberculosis var bovis enveloped viruses |
| What is the reactivity of Chloroxylene (PCMX) against bacteria |
less active against P. aeruginosa BUT increase activity against Pseudomonas spp and other pathogens ; |
| What bacteria easily grows in quats (household products), hot tubs, hospital tubing |
| Pseudomonas aeruginosa |
