question

3. Upon entering the lab each day, the following tasks must be performed in this order:

answer

a. Wash hands
b. Don lab coat
c. Put on gloves
d. Disinfect the bench

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leaving the lab perform what activities in reverse order

answer

disinfect bench tops
take off gloves
take off coat
wash hands

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a small glass tube that liquid is drawn into so that it can be measured, often b4 it is delivered to another container

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pipette

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Never place any instruments or materials into your mouth. (i.e. Do not pipette by
mouth. Use the

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mechanical apparatus provided

question

. Cultures must always be carried in a __________ when moving around the laboratory

answer

test tube rack

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b. Cultures must be kept in a _____________ on the bench tops when not in use.

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test tube rack

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Broth cultures must never be __________ by mouth. Always use a suction aid (never use your mouth) when filling a pipette or use a pipettor with a biological or chemical reagent

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pipetted

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Broken glass, used slides, pipettes, and swabs should all be disposed of in the

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sharps container

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Gloves should be disposed of in the ___________. Gloves NEVER go in the
normal trash cans.

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used gloves receptacle

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Label all materials with

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name, date and the organism that is being cultivated.

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To avoid contamination, all instruments such as loops and needles should be sterilized by ______

. Nothing used for the transfer of bacteria should ever touch
the bench tops.

answer

incineration

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Sterilization techniques will involve the use of __________ that are fire and burn hazards. _________ reach an internal temperature of 850 ?C (1500 ?F). Keep all combustibles away from the _______.
a. Do not leave inoculating loops or needles propped in the ___________.
Take caution with loops and needles. They should never be waved to cool

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Bacticinerators

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7. Proper procedure for transferring cultures should always be followed:

_______ loops/needles before use.

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sterilize

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7. Proper procedure for transferring cultures should always be followed:

. properly remove the caps to tubes- so the rim is not touching your

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glove

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7. Proper procedure for transferring cultures should always be followed:

Never place caps on the

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bench tops

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7. Proper procedure for transferring cultures should always be followed:
Only hold ______ tube at a time

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one

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7. Proper procedure for transferring cultures should always be followed:

Bring the liquid to

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you

question

7. Proper procedure for transferring cultures should always be followed:

Only insert the portion of the ___________ that got red hot in the
Bacticinerator (this is the only portion of the _______ that is sterile)

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loope nor needle

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7. Proper procedure for transferring cultures should always be followed:

Replace the cap and put the tube back in the ________

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rack

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7. Proper procedure for transferring cultures should always be followed:
_______ the loop/needle before returning it to its container.

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re-stirilize

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. Observe _______ technique at al times when dealing with microbial cultures

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aseptic

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aseptic

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free from the living germs of disease, fermentation, or putrefaction

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10. Place all cultures in the appropriate __________ or in designated waste areas.

answer

incubators

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You will be responsible for the proper care of any microscope that you are using.
Make sure that you _______ each microscope that you get and that you inform the instructor of any issues with the microscope.

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sign out

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All microscopes must be _______ before and after use.

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cleaned

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Oil immersion should only be used with the ____________
always rotate the objectives in a clock-wise fashion to avoid exposing other objectives to the oil.

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100x oil immersion objective

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5. Procedure For Cleaning Microscopes:

Move the stage to the ______________. Use the ______ adjustment knob to obtain maximum working distance and remove the slide from the stage.

answer

lowest position

coarse

question

5. Procedure For Cleaning Microscopes:

turn off th __ and _____________ the cord. store _ appropiately.

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light

cord

question

5. Procedure For Cleaning Microscopes:

Using lens paper, clean all the lenses starting with the cleanest first ___ ____ ______ and ______. use lens cleaner if necessary

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ocular, low power, high power and oil immersion.

question

5. Procedure For Cleaning Microscopes:
Clean any oil off of the stage using

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Kimwipes

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5. Procedure For Cleaning Microscopes:
rotate the __________ into place.

answer

scanning objective (4x_

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label culture vessels w/ non-water soluable gass marckers and or self stick labels prior to

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inuculation

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place the lABELING DIRECTLY BELOW THE ___________ of the culture tube

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cap

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when labeling a petri disn only the ____________ of the organism should be written on the ________ of the plate, close to its perphery to prevent itsobscuring observation response.
add info should be written on cover

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name

bottom

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to avoid killing the cells and splatering the culture, cool the inaculating wire by ____________________________________ cover prior to obtaining inoculum, or touch the edge of the medium plate

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tapping the inner surface of the culture tube or petri dish

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if an ______ culture is used , only tough a single area of growth w/ the inucolating wire to obtain the inoculum

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agar

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never ___________ the loope or needle over the entire surface, and take care not to dig in the solid medium

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drag

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if a broth medium is used first tap the bottom of the tube against the palm your hand to suspend

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the microorganism

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do not tap culture vigourously as this may cause spills or excessive foaming of the culture which may

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denature the proteins in the medium

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when using a pipette, use a _____- to obtain and deliver the material to be inoculated

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mechanical pipetting device

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in incubation optimum growth occurs @ ______ over a period of 18 to 24 hrs

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37c

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petri dishes must always be incubated in an ____________ to prevent water condensation from dropping onto the surface of the culture medium, producing confluent microbial growth

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inverted position (top down)

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a culture containing a single unadulterated species of cells id called a

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pure culture

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for survival, most microbes ,must use soluable low molecular weight substances that are frequently derived from enzymatic degradation of complex nutrients. a solution containing these nutrients is a

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culture medium

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a liquid nedium lacks a solidifying agent and is called a

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broth medium

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a broth medium supplemented w/ a solidifying agent called _____________ results in a solid or semi-solid medium

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agar

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an extract of seeweed is a complex carbohydrate compossed mostly of galactose, no nutitional value.excellent solidifying agent cause liquifies @ 100 c and solidifies @ 40c

answer

agar

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a completely solid medium requires an agar concentration of

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1.5 to 1.8

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a solution of less than 1% agar results in a

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semisolid solution

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while in a liquified state, solid media can be placed in test tubes, which are allowed to cool or harden in a slanted position producing

answer

agar slants

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agar slants are useful for mainting pure cultures. similar tubes that, following preparation, are allowed to harden in an upright position

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agar deep tubes

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used gaseous requirements for microorganisms

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agar deep tubes

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agar deep tubes may be liquified in a boiling water bath and poured into petri dishes
provide surface area for the study od microorganisms

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agar plates

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the process of a rendering a medium or material free of all forms of life

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sterilazation

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glass ______- and glass and or plastic __________ are used to cultivate microoraganisms

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test tubes
petri dished

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a suitable medium in the form of broth may be used in the test tube but only a ___________ is used in petri dishes

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solid medium

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provide a larger surface area for growth and cultivation. bottom portion contining the medium
top serves as a loose cover

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petri dish

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must be carried under aseptic conditions
microorganisms must be transferred from one vessel to another or from stock cultures to various media for maintenence and study

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subculturing

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used for aseptic techniques
similar in function to straws (draw up liquid)
the are calibrated to deliever diff volumes depending on requirements.
may be sterilized inside bulk canisters
or wrapped individually in brown paper bag and sterilized in autoclave or dry heat oven

answer

pipette

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used to cultivate microorganisms
provides a rapid uniform transfer of heat to culture vessel, and its agitation provides increased aeration, resulting in acceleration of growth
instrument can only be used in broth medium

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shaking waterbath

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how should microscopes be handle

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hold suppot base by one hand and grip the neck w/ the other

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when one lens is in focus the other lens will also have the same focal length and can be rotated into position w/out major adjustment

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parafocal

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what is the scanning lens magnification

answer

4x

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can be used to describe any bacterium that has a spherical shape

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coccus

[image]

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rod-shaped bacteria and a member of the division Firmicutes

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bacillus

[image]

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is a genus of Gram-negative bacteria possessing a curved rod shape,several species of which can cause foodborne infection, usually associated with eating undercooked seafood

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vibrio

[image]

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belong to a phylum of distinctive Gram-negative bacteria, which have long, helically coiled (spiral-shaped) cells

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spirillum/spirochetes

[image]

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is a genus of Gram-positive rod-shaped bacteria and a member of the division Firmicutes

answer

[image]

individual bacillus

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diplobacil´li a short, rod-shaped organism occurring in pairs. ...

answer

diplobacillus

[image]

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is a genus of aerobic, gram-negative facultative anaerobe bacteria, which grow in culture as rods in chains

answer

stretobacillus

[image]

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is a round bacterium (a coccus) that typically occurs in pairs of two joined cells

answer

diplococcus

[image]

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is a genus of spherical Gram-positive bacteria belonging to the phylum Firmicutes^[2] and the lactic acid bacteria group. Cellular division occurs along a single axis in these bacteria, and thus they grow in chains or pairs

answer

streptococcus

[image]

question

is a genus of Gram-positive bacteria. Under the microscope they appear round (cocci), and form in grape-like clusters.^[1]

answer

staphylococcus

[image]

question

4

[image]

answer

tetrads

[image]

question

cocci shaped bacteria that fail to dissociate during replication such that they form a cube of eight bacteria.

answer

sarcinae

[image]

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