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Micro Lab Practicum Slides – Flashcards

Michael Seabolt
7 July 2022

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Amoeba
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Entamoeba cyst
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Entamoeba trophozoite
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Giardia
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Paramecium
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Plasmodium
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Trichomonas vaginalis
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Trypanosoma
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Chlamydomonas
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Diatoms
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Dinoflagellates
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Euglena
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Spirogyra
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Volvox
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Scenedesmus
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Aspergillus
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Candida
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Coprinus
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Penicillum
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Penicillum
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Rhizopus
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Saccharomyces
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Lichen
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Lichen
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Ascaris
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Ascaris eggs
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Clonorchis
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Clonorchis eggs
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Dipylidium
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Dipylidium pisiforms
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Dipylidium pisiforms
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Dipylidium eggs
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Echinococcus
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Enterobius
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Enterobius eggs
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Fasciola
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Fasciola
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Fasciola
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Necator eggs
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Paragonimus
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Paragonimus eggs
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Schistosoma mansoni cercariae
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Schistosoma mansoni female
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Schistosoma mansoni male
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Strongyloides
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Taenia
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Taenia pisiforms
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Trichinella
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Trichinella tissue infection
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  • Nematode
  • Male 8-11 inches, curled tail
  • Female 10-14 inches, straight tail
  • eggs: rough surface
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Ascaris
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  • Nematode
  • Adult: curved neck & hooked mouth
  • Tail: pointed
  • Eggs: rough shaped mass inside smooth membrane
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Necator
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  • Nematode
  • Unstainted samples
  • Male: curved tail
  • Female: straight with pointed ends
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Strogyloides stercoralis
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  • Nematode
  • Stained Pink
  • Curved body
  • Infect muscle tissue
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Trichinella
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  • Male: 2-5 mm, blunt tail
  • Female: 8-12mm, pointed tail
  • Unstained
  • Eggs: Flattened on one side
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Enterobius vermicularis
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  • Hermaphroditic
  • Adult: 10-25mm with long "snout"
  • Eggs: oval, one end more pointed than other, hook at round end
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Clonorchis
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  • Adult: ~ 1cm long
  • Infect humans through undercooked seafood
  • Stained pink
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Paragonimus
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  • Adult: broad and flat, up to 30mm long, 15mm wide
  • Anterior end is cone shaped
  • Infect aquatic snails and water plants before reaching mammalian hosts
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Fasciola hepatica
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  • Adult female: Thin, stained pink, reside in gynecophoral canal of males.
  • Adult male: thicker than females, stained pink
  • Eggs: 120 - 180um prominent lateral spine
  • Cercariae: prominent head with pronged tail
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Schistosoma mansoni
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  • Hooked scolex
  • 7-13 gravid uterine branches
  • Produce 50,000 eggs per proglottid
  • Eggs: 30 - 35 um, radially striated
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Taenia solium
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  • No hooks on scolex, wider than pork variety
  • 15 - 20 gravid uterine branches per proglottid
  • Eggs: 30-35 um, radially striated
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Taenia saginata
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  • Extended "sucker" on scolex
  • Proglottids shorter than human variety
  • Eggs: appear to have multiple circles inside
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Dipylidium canium
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  • Hooked scolex with suckers
  • Fewer proglottids than Taenia and Dipylidium
  • Slide presents ~3 proglottids
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Echinococcus
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  • Protozoa
  • Cysts: Two to three nuclei visible, and chromatoid bodies
  • Trophozoites: Passed through stool 
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Entamoeba histolytica
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  • Protozoa
  • Trophozoites: ear-shaped, 10-20 um, 2 nuclie, larger & pointed at one end
  • Cysts: oval to ellipsoid, 8-19 um, 2 nuclei
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Giardia lamblia
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  • Pear-shaped oval structure
  • No cyst form
  • Barb-like axostyle at the base
  • Stained pink
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Trichomonas vaginalis
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  • Protozoa
  • Presented in red blood cell smear
  • Worm-like body with motile "taile
  • Stains pink
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Trypanosoma brucei
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  • Protozoa
  • Present within red blood cells as ring-formed trophozoites
  • Replicate through extracellular gametocytes after RBC bursts
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Plasmodium falciparum
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  • Fungi
  • Stains blue
  • Striated circular cross section with canal in center
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Coprinus
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  • Fungus
  • Named for liturgical "holy water sprinkler"
  • Stem-like shape with blooming cluster formation at head
  • Stains streaks of teal with dark blue masses
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Aspergillus
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  • Fungus
  • Presents as clusters of unstained ovals
  • Produce ascopores
  • 20 um in diameter
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Saccharomyces
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  • Fungus
  • Presents as unstained ovals, more transparent than Saccharomyces
  • Can form budding stalks
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Candida
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  • Fungus
  • Filamentous branches terminating in spore clusters
  • Present with green background and dark blue branches
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Rhizopus
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  • Fungus
  • Filamentous, violet stained with numerous branches
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Penicillium
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  • Fungus
  • Crust-like spreading masses
  • Can be violet or teal stained
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Lichen
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  • Algae
  • Wide diversity of form, but bilaterally symmetrical
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Diatoms
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  • Algae
  • Unicellular flagellate, ellongated oval shape
  • Stain teal/green with central nucleus
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Euglena
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  • Algae
  • Filamentous, spiral or helix of chloroplasts
  • Long, rectangular formations
  • Stained dark violet
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Spirogyra
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  • Algae
  • Round shape, eye-like spot in anterior portion
  • Unstained with thick cell wall presenation
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Chlamydomonas
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  • Algae
  • Marine plankton
  • Present as large, transparent round cells with dark teal organelles
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Dinoflagellates
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  • Algae
  • Circular, symmetrical cells
  • Present as dark purple/blue clusters of cells
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Volvox
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What is the right structure for an essay
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A paragraph is a related group of sentences that develops one main idea. Each paragraph in the body of the essay should contain:
  • a topic sentence that states the main or controlling idea
  • supporting sentences to explain and develop the point you’re making
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