MasteringGenetics Ch15 HW – Flashcards
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Which term is used to describe enzymes that are produced only when specific substrates are present in the environment?
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inducible
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What is the role of the lacZ gene product, the enzyme ß-galactosidase?
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It converts lactose to glucose and galactose.
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Which of the following is an example of positive control in operons?
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the action of the catabolite activator protein (CAP) in the lac operon
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Which of the following mutations could lead to constitutive expression of the genes of the lac operon?
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A mutation in the operator sequence
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Which of the following best describes the biological role of the lac operon?
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It ensures that a cell dedicates resources to the production of enzymes involved in lactose metabolism only when lactose is available in the environment.
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The placement of the operator sequence between the promotor and the structural genes is critical to the proper function of the lac operon.
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true
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Which of the following statements best describes the trp operon?
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It is repressible and under negative control.
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The enzymes involved in lactose metabolism are
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needed when lactose is present.
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The enzymes involved in tryptophan metabolism are
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needed when tryptophan is absent.
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In positive control of gene expression,
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the regulatory molecule stimulates transcription.
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In negative control of gene expression,
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removal of the regulatory molecule from DNA stimulates transcription.
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Line (a)
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The repressor is bound to the operator, and CAP is bound to its binding site. The operon is not transcribed.
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Line (b)
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The repressor is inactive, and CAP is bound to its binding site. The operon is being transcribed at a high level.
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Line (c)
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The repressor is bound to the operator, and CAP is not bound to its binding site. The operon is not transcribed.
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Line (d)
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The repressor is inactive, and CAP is not bound to its binding site. The operon is transcribed at a low level.
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Under a system of positive control, transcription does not occur unless a regulator molecule directly stimulates RNA production.
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true
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The enzyme permease cleaves the linkage between glucose and galactose residues in lactose.
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false
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The lac operon consists of three structural genes as well as the adjacent region of DNA known as the regulator.
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false
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Which term most appropriately refers to a regulatory protein in prokaryotes?
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DNA binding protein
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Which of the following occurs as a result of an abundance of tryptophan in E. coli?
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The 5 trp genes (TrpA - TrpE) are not transcribed.
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Which of the following features of the trp operon is likely least essential to the process of attenuation?
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The order of the structural genes, E, D, C, B, A
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Attenuator systems such as the one described for regulation of tryptophan synthesis would be just as likely to occur in eukaryotes as in prokaryotes.
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false
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The trp and lac operons are both subject to forms of control that are typically called negative.
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true
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When referring to attenuation in regulation of the trp operon, it would be safe to say that when there are high levels of tryptophan available to the organism, ________.
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transcriptional termination is likely