MacConkey Agar – Flashcards

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question
selective media creates..
answer
gram negative bacteria
question
bile salts and crystal violet inhibit..
answer
growth of gram positive bacteria
question
media is used for what?
answer
isolate and differentiate enterobacteriaceae (lactose fermenters-so they ferment the lactose in macconkey media
question
what purpose does nutrient agar plate serve? what way does it increase the validity of the test results?
answer
gram negative will only grow on macconkey
question
with respect to macconkey agar what would be the possible consequence of: a. replacing lactose with glucose b. replacing neutral red with phenol red
answer
a. there wouldn't be a differentiation b. the acid producers would be yellow
question
who would removing crystal violet from macconkey agar alter the sensitivity and specificity of the medium?
answer
gram positive will be able to grow crystal violet inhibits growth of gram positive
question
compare the recipes of nutrient agar and macconkey agar if an organism can grow on both media on which would you expect it to grow better?
answer
best on nutrient agar because it provides lost of nutrients for the organism
question
macconkey agar is a selective medium it is also a defined or an undefined medium why is that formulation desirable?
answer
undefined beneficial by giving wide variety of growth ability
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