Experiment 1: Thin Layer Chromatography – Flashcards
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• What principle information is obtained by running Thin Layer Chromatography?
o Bond lengths
o Hydrogen bonding
o Functional groups present
o Ratio of compounds in the sample
o Stereochemistry
o Relative polarity of compounds
answer
relative polarity of compounds
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how to determine the relative polarity of spots based on their position on the TLC plate
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non-polar compounds will be at top, polar compounds on bottom
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Rf calculation for compounds on TLC plate
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distance spot traveled divided by distance solvent traveled. Higher the Rf value, the less it's polar
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• How could Thin Layer Chromatography be used to aid in the identification of a a compound
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Thin layer chromatography can be used to identify a compound because for certain reaction mixtures, TLC can separate all compounds in a mixture based on the distance the compound travels on the TLC film. With the proper solvent system, TLC can be used to uniquely identify a compound.
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• Which pair of compounds would TLC separate most easily? Why?
o Polar and polar
o Polar and nonpolar
o Nonpolar and nonpolar
answer
polar and nonpolar
