DNA Structure – Flashcards

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structure of DNA
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right-handed/double helix
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DNA and RNA are made of repeating units called
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nucleotides
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DNA stands for
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Deoxyribose Nucleic Acid
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the bonds that hold repeating units together in each strand of DNA
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phosphodiester bonds
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information contained in DNA is encoded by the
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nitrogenous bases on the inside of the DNA molecule
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direction DNA is read
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5' to 3'
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letters in genetic code of DNA (nitrogen bases)
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A,C,G, and T
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A
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adenine
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C
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cytosine
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G
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guanine
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T
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thymine
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A pairs with
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T pairs with
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G pairs with
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C pairs with
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make up nucleotides
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deoxyribose (5 carbon sugar), phosphate group, nitrogen base
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RNA
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ribonucleic acid
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nitrogen bases in RNA
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Adenine, Cytosine, Guanine, and Uracil
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supercoiling
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DNA is twisted beyond double helix
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semi-conservative
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right hypothesis to how DNA replicates by Watson and Crick. Proved by Menselson and Stahl.
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DNA ligase
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seals together small DNA pieces
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DNA polymerase
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builds new strands of DNA
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DNA helicase
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untwists and unwinds DNA
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3 hypotheses to DNA replication
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conservative, semi-conservative, disperitive
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3 steps of DNA replication
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initiation, elongation, termination
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DNA replication result
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two DNA molecules identical to the original molecule
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initiation
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helicase unwinds and untwists the double helix so replication can begin
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elongation
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new nucleotides are added in one continuous strand by DNA polymerase on the leading strand and in small pieces on the lagging strand
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termination
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RNA primers are removed and the gaps are filled in by DNA ligase on the lagging strand
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Photo 51
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image produced by Rosalind Franklin of DNA structure. delivered by Wilkins and interpreted by Watson and Crick as X = helix and the scatter = double helix
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X-ray crystallography
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crystallise protein, blast it with an x-ray which will get diffracted. Image is from the scatter.
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genome
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complete set of human genes, in 23 pairs of chromosomes
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chromosome
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one pair of very long DNA molecules
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enzymes
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proteins that catalyse the body's chemical reactions
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mutation
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mistakes in gene replication
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junk DNA
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majority of DNA is a jumble that doesn't spell out genes
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transcription
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process of making mRNA from DNA
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polypeptide
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long chain of amino acids that can be folded into functional protein
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RNA processing/splicing
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cell has transcribed all the DNA into the mRNA, producing pre-mRNA. Now, introns are removed, and extrons are kept and spliced together
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5'
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short RNA sequences added to the ends of the RNA to protect it and guide it to the right location
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DNA replication
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process of producing mRNA from DNA template
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RNA polymerase
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enzyme that adds nucleotides to the newly formed RNA molecule during transcription
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translation
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making a polypeptide from a mRNA sequence
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codon
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group of three letters, codes for a certain amino acid.
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stop codon
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instead of an amino acid, codes for a water molecule instead and stops the chain
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ribosomes
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composed of rRNA (ribosomal). Read coding and put amino acids together to form polypeptide.
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