CITI biohazard training – Flashcards

humans animal/plant pathogens prions genetically modified organisms/microorganisms potentially infectious human material (blood, body fluids, tissues, etc) known pathogens, pests, noxious weeds, arthropods and insects not indigenous

a biological agent or condition (as in an infectious organism or insecure laboratory procedures) that constitutes a hazard to man or his environment; also a hazard posed by such an agent or condition.

1, application of good microbiological work practices 2 , use of safety and containment equipment 3, consideration of secondary containment in the design of lab facilities

Group 1: don't drink it Group 2: don't touch it Group 3: don't breathe it Grou 4: don't do it

1, spectrum of known/potential hazards 2, additional risks generated by proposed in-lab manipulations 3, the individuals who can safely perform the work

1, word practices 2, sharp instruments 3, disinfection 4, Safe Pipetting and Miscellaneous Work Practices

1, Substitution (using an attenuated or defective strain of a pathogen instead of the wild type) 2, Administrative (only allowing the most experienced personnel to conduct high risk work) 3, Engineering Controls (requiring that all work be conducted within a biosafety cabinet) 4, Personal Protective Equipment (requiring researchers to wear a respirator for their research) 5, Work Practices (working safely and consistently to minimize exposure)

1, Do not eat, drink, or smoke in the laboratory. Do not store food in the laboratory. Keep your hands away from your face (avoid touching eyes, nose, or mouth with gloved hands). 2, Do not pipette liquids by mouth. 3, Wear personal protective clothing in the laboratory (such as lab coats, gloves, and face protection when splash or splatter is anticipated). 4, Minimize or eliminate (if possible) the use of sharp instruments. If required, work very carefully with sharps. 5, Work carefully to minimize the potential for aerosol formation. Confine aerosols as close as possible to their source of generation (in other words, by use of a biosafety cabinet). 6, Disinfect work surfaces and equipment after use. (See Section 5.3 of this Module.) 7, Wash hands after removing gloves and protective clothing, after contact with contaminated materials, and before leaving the laboratory.