Ch 3 multiple choice – Flashcards

Assume you stain Bacillus by applying malachite green with heat and then counterstain with safranin. Through the microscope, the green structures are A) cell walls B) capsules C) endospores D) flagella E) impossible to identify

Three dimensional images of live cells can be produced with A) darkfield microscopy B) flourescence microscopy C) transmission electron microscopy D) confocal microscopy E) phase contrast microscopy

Carbolfuchsin can be used as a simple stain and a negative stain. As a simple stain the pH is A) 2 B) higher than the negative stain C) lower than the negative stain D) the same as the negative stain

Looking at the cell of a photosynthetic microorganism, you observe that the chloroplasts are green in brightfield microscopy and red in flourescence microscopy. You conclude that A) chlorophyll is flourescent B) the magnification has distorted the image C) you're not looking at the same structure in the microscope D) the stain masked the green color E) none of the above

Which of the following is not a functionally analogous pair of stains? A) nigrosin and malachite green B) crystal violet and carbolfuchsin C) safranin and methylene blue D) ethanol acetone and acid alcohol E) none of the above

Which of the following pairs is mismatched? A) capsule- negative stain B) cell arrangement - simple stain C) cell size - negative stain D) gram stain - bacterial identification E) none of the above

Assume you stain clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endoscopes are _1_ , and the cells are stained _2_. A) 1- red; 2- black B) 1-black ; 2- colorless C) 1- colorless; 2- black D) 1- red; 2- colorless E) 1- black; 2- red

Assume that you are viewing a gram stained field of red cocci and blue bacilli through the microscope. You can safely conclude that you have A) made a mistake in staining B) two different species C) old bacterial cells D) young bacterial cells E) none of the above

In 1996, scientists described a new tapeworm parasite that had killed at least one person. The initial examination of the patients abdominal mass was most likely made using A) brightfield microscopy B) darkfield microscopy C) electron microscopy D) phase contrast microscopy E) fluorescence microscopy

Which of the following is not a modification of a compound light microscope? was most likely made using A) brightfield microscopy B) darkfield microscopy C) electron microscopy D) phase contrast microscopy E) fluorescence microscopy