Biology Lab Manual #3 – Flashcards
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carbohydrates, proteins, lipids and nucleic acids
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the four basic classes of large organic molecules (macromolecules)
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monosaccharides (simple sugars)
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building blocks of carbohydrates
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amino acids
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building blocks of proteins
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fatty acids
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building blocks of lips
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nucleotides
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building blocks of nucleic acids
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unknown
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what is being tested
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known compounds/controls
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used for comparison to identify an unknown
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positive control
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produces a positive reaction
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negative control
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does not contain the variable for which you are searching and does not react in the test (water is a good example) and produces a negative reaction
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glucose, fructose, galactose
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examples of monosaccharides
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sucrose, lactose, maltose
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examples of disaccharides
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oligosaccharide
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a carbohydrate molecule that contains more than 2 and less than 10 sugar units
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polysaccharide
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generally has more than 10 sugar units
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starch
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a polysaccharide composed of numerous glucose units linked together
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benedict's test
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detects the presence of reducing sugars
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most monosaccharides and a few disaccharides
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benedict's test detects the presence of most __________ and a few __________
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blue; orange; red-orange
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when a reducing sugar reacts with the benedict's reagent, the solution turns from a ____ color to an ________ or ____-______ color often with a typical brick-red precipitation
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iodine test
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test that detects the presence of starch
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pale yellow; blue-black
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the iodine test detects the presence of starch by changing color from a ____ ______ to a ____-_____, when iodine complexes with a starch molecule
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proteins
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polypeptides that have spontaneously folded into a characteristic 3D shape essential for normal protein functioning
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polypeptide
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long linear chains of amino acids
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albumin
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refers to any water soluble proteins, which are abundantly present in many biological substances, such as animal body fluid and egg whites
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bovine serum
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used as a pure protein sample in bradford test
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bradford test
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test that indicates the presence of proteins
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reddish brown; blue
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the bradford tests indicates the presence of proteins via a color change from _____ ____ to a _____
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no
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do amino acids or peptides react with the bradford test?
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diffusion
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the movement of molecules from an area of high concentration to an area of low concentration
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osmosis
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the diffusion of water through a selectively permeable membrane
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selectively permeable membrane
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a thin layer of material that contains holes of various sizes or pores
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selective permeability
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means that small substances and fluid, such as sugars, salt and water, can move freely in either direction across the membrane but the movement of larger substances are prevented
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high; low
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osmosis is a special case of diffusion where water moves across the selectively permeable membrane from a side of ____ water concentration to the side of _____
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dialysis
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an application of the semi-permeable membrane in medicine;used as an artificial replacement fro kidneys that fail to adequately filter waste products from the blood