Bio chapter 17 – Flashcards
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Which of the following variations on translocation would be most disadvantageous for a cell
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Translating polypeptides directly from DNA
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Garrod hypothesized that inborn erros of metabolism such as alkaptonuria occur because
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Metabolic enzyme to require vitamin cofactors and affected individuals have significant nutritional deficiencies
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How might PKU affect the presence or absence of alkaptonuria
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It would nave no effect because tyrosine is also from the diet
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The nitrogenous base adenine is found in all members of which group
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ATP RNA DNA
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A particular triplet of bases in template strand of DNA is 5 AGT 3, corresponding codon for mRNA is
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3 UCA 5
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The genetic code is essentially the same for all, what can be assumed
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A gene from an organism can theoretically be expressed by any other organism
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The universal genetic code is now know to have exceptions, evidence is found if which is true
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If UGA usually a stop codon is found to code for an amino acids such as tryptophan (usually UGG)
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Which represents a codon
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A triplet in the same reading from as an upstream AUG
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Provides evidence that RNA evolved from DNA
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DNA polymerase uses primers usually made of RNA
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Describes termination of transcription of prok
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RNA polymerase transcribes thorugh the termination sequence causing the polymerase to separate form the DNA and release the transcript
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Does not occur in prok eukaryotic gene express but in euk
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A poly a tail is added to the 3 end of the mRNA and a cap is added to the 5 end
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Which of the following is most probable advantage of organisms of such sigma switching
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It might allow polymerase to recognize different promoters under certain environmental conditions
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Which of following is function of poly a tail
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It codes for a sequence in eukaryotic transcripts ath signals enzymatic cleavage 10 to 35 nucleotides away
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What type of RNA polymerase is involved in transcription of mRNA for globulin protein
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RNA polymerase II
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Transcription in eukaryotes requires which of following in addition to RNA polymerase
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Several transcription factors
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TATA box highly conserved in evolution which of following does it illustrate
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Any mutation in the sequence is selected a aginst
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TATA sequence is found only several nucleotides away from start site of transcription. Relates to which of the following
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The number of hydrogen bonds between A T in DNA
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Ribozyme
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An RNA with enzymatic activity
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A transcription unit 8000 long use 1200 nucleotides to make a protein of 400 aa this is explained by
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Many no coding stretches of nucleotides that are present in mRNA
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During splicing which component of splices me catalyze so excision
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RNA
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Alternative RNA splicing
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Can allow the production of proteins of different sizes from a single mRNA
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In structural organization of many eukaryotic genes individual exons may be related to which of the following
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The various domains of the polypeptide product
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Student inserts mRNA in eukaryotic cell after removing 5 cap and Poly a tail, what would he find
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The molecule would be digested by restriction enzymes
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Which components of previous molecule will also be found in mRNA in cytosol
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5 UTR E1 E2 E3 E4 UTR 3
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When spliceosome binds to element of this structure where does it attach
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To the end of an intron
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Which of the following is a useful feature of introns for this model
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Introns allow exon shuffling
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Suppose exposure to chemical mutagen results in change in sequence that alters 5 end of intron 1 what may occur
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Inclusion of I1 in mRNA
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Suppose induced mutation removes most of the 5 end of the 5 UTR what may occur
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Removal of 5 UTR also removes the 5 cap and mRNA will quickly degrade
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Articulate triplet of bases in coding sequence of DNA is AAA the anticodon on the tRNA that binds the mRNA codon is
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UUU
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Accuracy in the translation f mRNA into primary structure of a poly peptide depends on specificity in the
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Bonding of the anticodon to the codon and the attachment of amino acids to tRNA
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What is function of GTP in translaton
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GTP energizes the formation of the initiation complex using initiation factors
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A mutant bacterial cell has defective aminoacyl sun taste attaches to tRNA with AAA instead of phenylalanine. The consequence is
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Proteins in the cell will include lysine instead of phenylalanine at amino acid positions specified by codon UUU
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There a 61 mRNA codons that specify an amino acid but only 45 tRNA
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The rules for base parking between the thrid base of a codon and tRNA are flexible
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Is first event to take place in translation in eukaryotes
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The small subunit of ribosome recognizes and attaches to the 5 cap of mRNA
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Which is function of signal peptide
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To translocation polypeptides across the ER membrand
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When translating secretory of membrane proteins ribosomes are directed to the ER membrane by
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A signal recognition particle that bring ribosomes to a receptor protein in the ER membrane
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An experimenter has altered the 3 end of tRNA corresponding to the amino acid methionine in a way that removes the 3 AC
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Methionine will not bind
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The process of translation whether in prokaryotes or eukaryotes requires tRNA aa ribosomal subunit and what
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Polypeptide factors plus GTP
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When ribosome reaches a stop codon on the mRNA no corresponding tRNA enters the A site if translation reaction was stopped at this point which would be possible to isolate
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An assembled ribosome with a polypeptide attached to tRNA in the P site
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What is the function of the release factor
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It binds to the stop codon on the A site in place of tRNA
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When the function of the newly made polypeptide is to be secreted from the cell where it has been made what must occur
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It's signal sequence must target it to the ER from whic it goes to teh Golgi
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Formation of tRNA is mutated attaches to same aa but anticodon loops has sequence AAU binds to UUA
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One mutated tRNA molecule will relatively inconsequential because it will compete with many normal ones
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Why might a point mutation in DNA make a difference in the level of protein activity
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It may substitute and amino acid in active site
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MUller, why do we test for its ability to induce mutation
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We want to prevent any increase in mutation requency
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Which type of mutation has most serious effect of product
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Deletion of two nucleotides
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Effect of nonsense mutation in gene
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It introduces a premature stop codon into the mRNA
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A farm shift mutation could result form
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Insertion or deletion of base
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Which Italian is most likely to be damaging to the protein it specifies
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A base pair deletion
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Which small scale mutation would most likely have bad effect on function protin
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Base deletion near start of gene
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Common occurring mutation in people with CF is deletion of single codon
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A poly peptide missing aa
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Which mutation will cause phenotypic change
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Single nucleotides deletion in exon coding for active site
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Which number of exons below is gene likely to have
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8
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True about protein synthesis prok
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Translation can begin while transcription is still in progres
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Most current description of gene
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A DNA sequence that is expressed to form a function product either RNA or polypeptide
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Which way is it most like the domain Euk
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Domain Arch have numerous transcription factors
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Which is true of transcription in Arch
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Roughly simultaneous with translation
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Comparing DNA and RNA replication in same cell which is true only of replicaitn
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Entire template molecule is represented in the product
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In order for Euk gene to be make into bac colony what must be included in addition to coding exons
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Bacterial promoter sequence
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When genome includes 20000 protein coding regions what doe it imply
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There are also genes for RNA other than mRNA
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According to Beadle and Tatum how many genes
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2
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A mutation results in a defective enzyme A which would be a consequence
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Accumulation of A and no production of B and C
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If A B C are all required for growth a strain that is mutant for the gen encoding enzyme A would be able to grow on which meia
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Minimal medium supplement with nutrient B only
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If A B C are all required for growth a strain mutant for the gene encoding enzyme B would be capable of growing on which media
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Minimal medium sub lamented with C only
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Possible sequence of nucleotides in template strand of DNA that would code for polypeptide sequence Phe leu ile val
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3 AAA GAA TAA CAA 5
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What amino acid sequence will be generated based on 5 AUG UCU UCG UUA UCC UGG 3
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Met ser ser leu ser leu
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Peptide has sequence NH2 Phe pro lys gly Phe pro COOH
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5 TTT CCC AAA GGG TTT CCC 3
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DNA template strand 5-3 complementary 3-5, given locally unwound double strand above in which direction does RNA polymerase move
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3 to 5 along template strand
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In transcription event of previous DNA where would the promoter be located
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To the right of the template
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The di peptide taht will form will be
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Proline threonine
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The anticodon loop of the first tRNA that will complement this mRNA is
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3 GGC 5
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What type of bonding is responsible for the maintaining the shape of tRNA molecule
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Hydrogen bonding between base pairs
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The figure represents tRNA that recognizes and binds a particular amino acid in the instance phenylalanine which codon on mRNA strand codes for this amino acid
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UUC
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Has its 3 end projecting beyond its 5 end what will occur at 3 end
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The amino acid binds covalently
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Add polynucleotide phosphorylase to solution of ATP and GTP how many artifical mRNA 3 nucleotide codons would be possible
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8
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Add to solution of ATP GTP UTP how many artificial mRNA 3 nucleotide codons would be possible
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27
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Where does tRNA 2 move to after this bonding of lysine to the polypeptide
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E site
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Which component of the complex described enter the exit tunnel through the large subunit of the ribosmes
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Newly formed polypeptide
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In Euk cells transcription cannot begin until
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Several transcription factors have bound to the promoter
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Not true of a codon
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It extends from one do end of a tRNA molecule
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The anticodon of a particular tRNA molecule is
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Complementary to the corresponding mRNA codon
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Not true of RNA processing
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Exons are cut out before mRNA leaves the nucleus
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Which component is not directly involved in translation
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DNA
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Which mutation would be most likely to have harmful effect on organism
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Single nucleotide insertion downstream of and close to the start of the coding sequence