The Hot Zone: Part 1 – Flashcards

loner, Frenchman, expatriate in Africa, amateur naturalist, 56 years old, medium height and build, straight brown hair, only close friends were women that he paid *ahem*, not much is known about him, now dead, worked at Nzoia Sugar Factory in western Kenya that spread along the Nzoia River, taking care of the water pumping machinery, came to Africa in 1979 just as HIV started, observed animals on weekends, had a house keeper named Johnnie who cleaned up and prepared meals, came down with the deadly Marburg virus and died in Intensive Care at Nairobi Hospital

Considered on of the best young physicians at the Nairobi hospital, he cared for Monet until his death, later comes down with the Marburg virus as well, but he makes a slow full recovery and survives

Examined Dr. Musoke and recommended the surgery

The head surgeon to operate on Doctor Musoke

Anonymous, the monkey inspector, knew his boss was releasing sick monkeys on an island, key witness in the Monet/Musoke case but was never questioned. The Entebbe monkey trader

Vetrinarian in the US Army, mother of two, wife, knows martial arts, auburn hair, green eyes, homecoming queen, has hands “too quick” to handle delicate work in dangerous situations, does all of the housework, doesn’t have much time to cook, so she did this on Saturday’s and reheated it during the week, hated blood, has a specialty in the effects of Biosafety Level 4 hot agents, works with Eugene Johnson to study Ebola and Marburg as his pathologist, finds a leak in her spacesuit while dissecting an infected monkey but is not thought to have the disease, promoted to Lieutenant Colonel, studied the effects of nerve gas on rats in Aberdeen, became chief of pathology at the Institute

oes research in AA-5, an expert on Ebola and Marburg, civilian researcher, reputation for being a bit wild, large man, broad heavy face, loose brown hair, brown beard, large gut, glaring deep eye, top-notch epidemiologist (studies viral diseases in the wild) but rarely publishes his work, shy, suspicious, aspired to find the hidden resevoir of Ebola, had nightmares of Ebola seeping through his gloves, studied blood serum from Peter Cardinal, proved that Marburg and Ebola can travel through air, couldn’t euthanize the healthy monkeys

Not related to Gene, soft-spoken, Nancy’s immediate superior, the one who decedes to put Nancy with Ebola, trains Nancy on how to handle Level 4 hot agents, appointed head of pathology at Walter Reed Army Medical Center

The first identified case of Ebola Sudan, storekeeper in a cotton factory in Nzara, salaried man, worked in a back office where bats roosted, died from Ebola on a cot in his compound, never went to the hospital, given a traditional Zande funeral, his grave is still visited by doctors who wish to view it, pay their respects and reflect on its meaning, known as a quiet remarkable man, no photos of him, not well known, perhaps tall and slender like his brother

lots of friends, a few mistresses, came down with Ebola Sudan, worked in the same office as Yu. G.

first to be diagnosed with Marburg, broke out on Aug 8, 1967 and died 2 weeks later

Infected with Marburg virus from killing an infected monkey

Infected with Marburg from breaking a dirty test tube

Nun at Yambuku hospital, breaks with Ebola Zaire and is taken to Kinshasa and dies there, religion prohibited an autopsy

Nun at Yambuku Hospital who assists a priest in taking Sister M.E. to the Ngaliema Hospital in Kinshasa, comes down with Ebola Zaire soon after

Nurse at the Ngaliema Hospital, cared for Sister M.E., gets Ebola Zaire but is in denial, came from a poor ambitious family, got a scholarship to go to college in Europe, walks all over town while infected to get medical help, had slow, constant bleeding, had 3 blood transfusions, did of a heartattack

President of Zaire, enforces his army to isolate the Bumba Zone and ensure that no one that could potentially be infected gets out, lends his airplane to researchers to study in Bumba

Not related to the other Johnsons, virus hunter, analyzed the nuns blood, NAMED EBOLA, flew to Zaire to study Ebola, chief of an international WHO team that gathered in Kinshasa to try to control Ebola, found viruses to be beautiful, organized a floating hospital ship in Bumba.

Married to Karl Johnson, ran tests on Ebola that classified it as different from Marburg

CDC doctor, virologist who helped identify the Marburg virus, one of the world’s leading electron-microscope photographers of viruses

went to Zaire with Johnson, went to Bumba as a member of a field exploration team, scared of landing in Bumba, Michigan’s state epidemiologist

Cared for Mayinga N. during her hospital stay, wore a gas mask at first but soon gave up and worked with Mayinga face to face, felt her grown children were no obligation and that she would likely die from this virus as well

Student at a boarding school in Denmark, visited his family in Africa, blonde hair, blue eyes, tall, thin, ten years old, in Mombasa when he developed red eyes, rushed to Nairobi Hospital, suffered from puffy lungs, bled out under his skin, died of Marburg, thought to have gotten infected while in Kitum Cave, amateur geologist, parents worked for a relief organization in Kenya, close to his sister

huge, solitary, extinct volcano, 14 thousand ft. tall, on the border of Uganda and Kenya, near to Sudan, covered in rainforest

Headache begins on the seventh day, then severe back pain, nauseation, fever, vomitting, dry heaves, eyes become paralytic, sullen, yellowish skin with red speckles, memory loss, “vomito negro”, skin begins to liquify, virus cells attempt to turn host into themselves, many blood clots which lead to stroke and brain damage. The final stage occurs when the victim has “crashed and bled out”. That is when they puke up blood, go unconcious, keep puking until their intestines rip open and they bleed from all orfices while the virus looks for a new host. tends to aim at eyes and testicles. Has strange effect on brian by making patients sullen, slightly aggressive, negativistic, or psychotic.

1967 erupted in Germany. Originated from Uganda. Unknown infected monkeys shipped to England and then to Germany for profit by Mr. Jones
Sick monkeys were said to be killed but really released onto a small island in Lake Victoria
Monkeys were trapped somehwere in the Sese Islands (isle of plagues): known for low-lying forested archipelago in the northwestern part of Lake Victoria, an easy boat ride from Entebbe
If the monkey trader was short of monkeys, he would go to the island and catch some to ship to Europe
The Marburg virus may have been burning through rural areas in Uganda not far from Kitum Cave
Hidden outbreak of the virus occurred on the slopes of Mount Elgon

fishing village outside Sese islands, Lake Victoria. One of first places in the world where AIDS appeared

United States Army Medical Research Institute of Infectious Diseases

Named after the Ebola River, a tribune of the Congo River, Ebola Zaire is the most lethal strain, Ebola Zaire first occured in Sept 1976

attacks every organ and tissue in the human body except skeletal muscle and bone. Transforms virtually every part of the body into a digested slime of virus particles. Related to measles, mumps, rabies, certain pnuemonia; made of seven proteins three of which are vaguelly understood while the other four are unknown, creates an explosive attack on your immune system, in 10 days has the effect of 10 years of AIDS, travels by direct contact with blood and bodily fluids. Hemorrhages under skin

small blood clots begin to appear int he bloodstream and the blood thickens and slows, and the clots begin to stick to the walls of blood vessels. Clots fit together in a mosaic.

property of microscope which allows objectives to be changed without having to refocus

of the objective (4x, 10x, 40x, 100x) times that of the eyepiece (10x)

distance between the objective and the object being viewed

thickness of specimen that an be seen in focus at one time

ability of the microscope to distinguish between two closely adjacent points. Measured in distance units (um micrometers). Given by formula d= wavelength of light (um)/ numerical aperture of objective + numerical aperture of condenser

0.9

1.25

0.55 (um)

measure of how much the speed of light is reduced inside a medium such as glass. The refractive index of air and glass are different enough so that light waves change direction when they cross the interface from air to the class. This necessitates the use of immersion oil with our 100x objective

blue top. red middle. yellow bottom

rods

spheres

spirals

rods with clear terminal swollen spores

spiral shapes

oval things will dots all around

chain of cocci

transfer microorganisms from one location to another without introducing contamination to the original culture

how most stocks are maintained and it is important to preserve this condition

lives in the shadow of Mount Elgon

Change favors the prepared mind

prominent cliff on the easter side of the volcano