Microbiology – Lab Questions – Flashcards

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1. The three physical forms of laboratory media are
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solid, semisolid, and liquid.
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2. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is
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inoculation
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3. Newly inoculated cultures must be _____ at a specific temperature to encourage growth.
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incubated
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4. A reducing medium contains
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substances that remove oxygen.
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5. Agar is an important component of media because
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agar provides a solid surface for bacterial growth.
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6. The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the
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inoculum
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7. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?
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The culture is contaminated
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8. A reducing media is used to culture
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anaaerobic organisms
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9. Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?
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transport
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10. A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible, would be termed
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chemically-defined
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11. What type of isolation technique is most effective for the majority of applications?
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streak plate
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12. A pure culture contains
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only one species of microorganism.
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13. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?
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The culture medium must be selective.
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14. Agar is a complex polysaccharide that comes from a/an
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algae
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15. For which bacterial genus does mannitol salt agar differentiate between species?
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Staphylococcus
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16. A nutrient medium that contains at least one ingredient that is NOT chemically definable would be termed enriched.
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complex
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17. Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?
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differential
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18. Bacteria that require special growth factors and complex nutrients are termed
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fastidious
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19. Which method often results in colonies developing down throughout the agar along with some colonies on the surface?
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pour plate
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20. A common medium used for growing fastidious bacteria is
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blood agar
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The ___ of the microscope holds and allows selection of the objective lens.
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condenser stage
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Which objective lens should always be used for viewing first?
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low power lens
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The coarse adjustment knob should never be used in conjunction with which objective lens?
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both the 40x and the 100x
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Once immersion oil is on the slide, never put the __ into place.
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scanning
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For cleaning and storing the microscope which objective lens should be in place?
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scanning lens
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