MCB3020 Final – Flashcards

The method of nucleotide sequence determination that utilizes dideoxynucleotides was developed by

Modern automated sequencing machines detect DNA bands by measuring incorporated radioactivity.

Dideoxynucleotide sequencing methodologies rely on the ability of dideoxynucleotides to facilitate chain extension after incorporation by DNA polymerase.

Each of the four Sanger sequencing reactions include

Most automated nucleotide sequencing machines detect bands by

The Sanger sequencing approach developed in the 1970s

Genomics is the study of

Automated nucleotide sequencing machines detect DNA bands by laser induced

After completing the nucleotide sequence of a microbial genome, computer translation of DNA sequence allows enzymes to be identified based on a short pattern of amino acid sequence called a contig that corresponds to the active site of the enzyme.

The whole-genome shotgun approach is useful for sequencing small bacterial genomes but has failed in the case of larger eukaryotic genomes such as those of Drosophila and human.

In genomic research the term contig refers to overlapping nucleotide sequences that are not adjacent in the genome and are formed from smaller fragments.

Clonal libraries used to determine the sequence of a microbial genome using the whole-genome shotgun approach are typically generated by

The whole-genome shotgun approach for collecting the data used to determine the nucleotide sequence of microbial genomes uses

An acronym that describes sequences of a protein-coding gene that specify the amino acid sequence is

The proteome refers to the collection of open reading frames in a genome.

ORF refers to

When two or more genes from the same genome have nucleotide sequences so alike that they most probably arose from gene duplication those genes are called

_______________ is the field concerned with the management and analysis of biological data using computers

Nucleic acids to be analyzed using microarrays are usually tagged by covalent attachment of ___________ molecules

______________ technology can be used to simultaneously monitor the level of expression of every gene in a cell

Expressed sequence tags are nucleotide sequences derived from cDNA molecules

DNA microarray technology is used to measure the

The genome sequence of Treponema pallidum

lacks numerous biosynthetic pathways and thus has numerous nutritional requirements

Analysis of the genome and transcriptome of the highly radiation resistant bacterium Deinococcus radiodurans has revealed all of the following except

The proteome refers to

Isoelectric focusing

Two-dimensional gel electrophoresis

Protein spots eluted from a 2 dimensional gel analysis can be identified and correlated to specific genes that code for them using

In modern proteomic analysis the amino acid sequence of a protein can be determined by

The proteome

Since proteins are encoded by nucleotide sequences, the proteome of a microorganism can be readily inferred from the given complete nucleotide sequence of a genome and a high speed computer with specialized software.

Bioinformatic analysis of genomic nucleotide sequences using specialized software and high-speed computers make it possible to determine how proteins encoded by ORFs are post-translationally modified.

The term "proteome" refers to the complete amino acid sequence of a protein.

Research focused on determining the function of different cellular proteins, protein interactions and protein regulation is called _________ proteomics.

Isoelectric focusing separates proteins based on the pH at which they are electrically

A long-term goal(s) of the bioinformatic analysis of a pathogenic bacterium is

In an experiment, you have created a microbial strain in which you have deleted a single gene; to evaluate the transcriptome and the proteome, you

Analysis of the genome of Mycobacterium tuberculosis has revealed all the following except

The subdiscipline of genomics that seeks to identify similarities and differences between genomes of different strains and species is called __________ genomics.

Genome analysis has revealed that horizontal gene transfer is frequently mediated by phages.

Which of the following is not a required characteristic of antigens from microbial pathogens that might be used in vaccine production?