Spectroscopy Flashcard

absorbance
(A) defined as A=log(P initial/P) where P initial is the radiant power of light striking the sample on one side and P is the radiant power emerging from the other side. Also called optical density
absorbance spectrum
a graph of absorbance or transmittance of light versus wavelength, frequency, or wavenumber
Beer’s Law
relates the absorbance (A) of a sample to its concentration (c), pathlength (b), and molar absorptivity (a), A=abc
calibration curve
a graph showing the value of some property versus concentration of analyte. when the corresponding property of an unknown is measured, its concentration can be determined from the graph
cuvet
a cell with transparent walls used to hold samples for spectrophotometric measurements
dilution factor
the factor used to multiply the initial concentration of reagent to find the diluted concentration
molar absorptivity
the constant of proportionality in Beer’s Law; A=abc (A is absorbance, a is molar absorptivity, b is pathlength, and c is the molarity)
spectrophotometry
in a broad sense, any method using light to measure chemical concentrations
transmittance
(T), defined as T = P/P initial were P initial is the radiant power of light emerging from the other side of the sample
wavelength
distance between consecutive crests of a wave

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