Lab report

The results were the following, the organic farm with a herd of 50 cows and a 10 acre field of zucchini had the highest levels of nitrites. Introduction: Blue Baby Syndrome Is a condition that affects many Infants. This condition makes the baby’s skin turn blue because of the lack of oxygen. This condition can exhibit lethargy, vomiting and not being able to breathe. It can even lead to death in rare cases. This condition is caused by the excess amount of nitrate that is then converted into nitrite by the digestive system. The hemoglobin then reacts with the nitrites to form Mothballing.

Mothballing is not a problem in adults since they have an enzyme that converts mothballing back to hemoglobin. Infants don’t have many of the enzyme to convert mothballing to hemoglobin, resulting In Blue Baby Syndrome. For example in Gopher Hollow there is a cluster of Blue Baby Syndrome. Four infants have been affected. The families of these infants all collect their water from a community well. Nitrates and Nitrites can be found in many things. For example in vegetables, Like carrots, lettuce and spinach. We do get many of our nitrogen from the food we eat but also through the water we drink. L) Levels above 10 pimp of Nitrate/ Nitrites are not dangerous but accumulations of it in soil and groundwater can cause high levels of these compounds. (l ) Causing high level of nitrites in drinking water and especially in wells. If a mountain lake that has become overgrown with algae then there could be an increase of Blue Baby Syndrome cases. The lake could be a mall water source. Methods: The first step In determining the culprit location Is to create a graph that represents he relationship between the amounts of nitrites in the solution and the optical density of standards of known concentrations.

These standards are the control group. Then a spectrophotometer was turned on was allowed to warm up for 1 5 minutes. There were nine standards to be tested using the spectrophotometer. One ml of each standard was labeled and placed In 13 by mm glass test tubes. The concentrations were: distilled water, 0. Pam, 1. Pam, 2. Pam, 4. Pam, 6. 0 pimp, 10. 0 indicator reagent ( 1 naphthylethylenediamine sulfanilamide) was added to each standard sample and mixed thoroughly. A new microcomputer tip was used to prevent mixing of chemicals.

The samples were left out to sit for five minutes to insure full color development. Then the spectrophotometer’s wavelength was set to Mann because the nitrite indicator absorbs light at Mann(1). I ml of the distilled water sample was transferred into a clean cavetti using a plastic transfer pipette. The cavetti was then placed inside the spectrophotometer . The button labeled “blank” was pressed and the ODD reading was 0. AAA . After getting the ODD, the blank sample was placed back into its original test tube. Then 1 ml of the 0. Pm standard was transferred into a clean cavetti and placed inside the spectrophotometer for ODD measurement. Once the measurement appears on the screen record it. This step was repeated for the remaining standard samples. After measuring the ODD each sample was transferred back into its original test tube. With the data collected, a graph was plotted with the Optical Densities on the vertical axis and the nitrite concentrations on the horizontal axis. Once the graph was plotted, a standard curve and the line of best fit were created and put on the graph. Whenever the ODD reading was above 1. It’s omitted from the graph because it means that there very light being detected. To get the line of best fit the equation y=mix+b was used and m=slope and b= y intercept. Then the unknown samples of the sites thought to be the cause of the Blue baby syndrome were tested using the same techniques as to find the standard samples. The first step, 2 ml of each water sample were place into four 13 by 85 mm glass test tubes. Then using a IPPP microcomputer, 40 micrometers of sulfanilamide was added to each test tube. The test tubes were allowed five minutes to develop color.