Chemistry Internship Study Questions Section 1

List the routine Chemistry tests MOST affected by hemolysis
K, LD, CK, ALP, AST, PO4, Iron
What routine chemistry results would be MOST affected by contamination with IV solution?
K, Glucose, Na, Cl
What effect will EDTA have on a calcium level?
Decreased
What effect will EDTA have on a Potassium level?
Increased
What effect will NaFl have on a BUN?
Decreased
What effect will NaFl have on Na?
Increased
What effect will NaFl have on K?
Increased
List the Critical Values (for adults) for the following analytes, Include reporting units:

Na

<120 mEq/L
>155 mEq/L
List the Critical Values (for adults) for the following analytes, Include reporting units:

CO2

<10
>40
List the Critical Values (for adults) for the following analytes, Include reporting units:

Calcium

<7 mg/dL
>12 mg/dL
List the Critical Values (for adults) for the following analytes, Include reporting units:

Ionized Ca

<0.75 mmol/L
>1.63 mmol/L
List the Critical Values (for adults) for the following analytes, Include reporting units:

K

<3 mEq/L
>6 mEq/L
List the Critical Values (for adults) for the following analytes, Include reporting units:

Phosphate

<1
>8
List the Critical Values (for adults) for the following analytes, Include reporting units:

Mg

<1 mg/dL
>9 mg/dL
List the Critical Values (for adults) for the following analytes, Include reporting units:

Glucose

<50 mg/dL
>450 mg/dL
What tests are included in a Basic Metabolic Profile (BMP)?
Na, K, Cl, CO2, BUN, Creatine, Ca, Glucose
What tests are included in a Liver Function Panel?
Bilirubin, ALP, AST, LD, GGT, Albumin
What tests are included in a Lipid Panel?
Cholesterol, Triglycerides, HDL, LDL
What minimum information should be included on EVERY specimen container?
Name, MRN, DOB, Date/Time, Collector
What is the Formula for Beer’s Law?
(Unknown Abs / Standard Abs) = (Unknown Concentration / Standard Concentration)
How would you calculate the concentration of the Unknown using Beer’s Law given the following information:

Unknown Abs = 0.028
Standard Abs = 0.019
Standard Concentration = 20.0 mg/dL

(0.028/0.019) x (20.0) = Unknown Concentration
Calculate the concentration of the Unknown using Beer’s Law given the following information:

Unknown Abs = 0.028
Standard Abs = 0.019
Standard Concentration = 20.0 mg/dL

29.5 mg/dL
What is the purpose of using specimen or reagent blanks in some spectrophotometric assays?
“Subtract” out absorbance from pigmented specimens or reagents
What is the function of a monochromator in a spectrophotometer?
Isolate specific wavelength
Name some common types of monochromators in a spectrophotometer.
Prisms, Filters, Diffraction gratings
In atomic absorption spectrophotometry, what is the light source?
Hollow cathode tube
What type of compound is usually being analyzed in atomic absorption spectrophotometry?
Trace Elements / Metals
Match the type of instrumentation to its principle of operation:

Electrophoresis

Separation of proteins based on charge and size
Match the type of instrumentation to its principle of operation:

Osmometry

Measures the total number of dissolved particles in a solution
Match the type of instrumentation to its principle of operation:

Amperometry

Current is measured in an electrochemical cell when a constant voltage is applied
Match the type of instrumentation to its principle of operation:

Nephelometry

Measures light scattered by ag-ab complexes
Match the type of instrumentation to its principle of operation:

Fluorometry

Measured substances that absorb light at one wavelength and emit light at a second, different wavelength
Match the type of instrumentation to its principle of operation:

Chemiluminescence

Chemical reaction that produces photons of light
Match the type of instrumentation to its principle of operation:

Potentiometry

Voltage difference measured between a reference and an indicator electrode; pH meters and ISE
Match the type of instrumentation to its principle of operation:

Chromatography

Separation of mixtures into individual components based on specific physical characteristics
What is the most common application of potentiometry in a routine clinical lab?
Ion selective Electrodes for Electrolytes
Match the principle of operation with its instrument:

Separation of proteins based on charge and size

Electrophoresis
Match the principle of operation with its instrument:

Measures the total number of dissolved particles in a solution

Osmometry
Match the principle of operation with its instrument:
Current is measured in an electrochemical cell when a constant voltage is applied
Amperometry
Match the principle of operation with its instrument:

Measures light scattered by AGN-ABY complexes

Nephelometry
Match the principle of operation with its instrument:

Measured substances that absorb light at one wavelength and emit light at a second, different wavelength

Fluorometry
Match the principle of operation with its instrument:

Voltage difference measured between a reference and an indicator electrode; pH meters and ISE

Potentiometry
Match the principle of operation with its instrument:

Separation of mixtures into individual components based on specific physical characteristics

Chromatography
Match the principle of operation with its instrument:

Chemical reaction that produces photons of light

Chemiluminescence
The following are examples of what type of instrumentation:
pH meters and ISE
Potentiometry
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